大豆GmCOL1和GmCOL13启动子和基因组基因的克隆与生物信息学分析  

Cloning and Bioinformatics Analysis of Promoters and Genomic Genes of GmCOL1 and GmCOL13 in Soybean

作　　者：韩英英[1] 陈福禄[1] 傅永福[1] 张晓玫[1] 

机构地区：[1]中国农业科学院作物科学研究所国家农作物基因资源与遗传改良重大科学工程农业部北京大豆生物学重点实验室,北京100081

出　　处：《生物技术通报》2017年第8期63-72,共10页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31371703;31570289);转基因专项(2016ZX08004-005;2016ZX08009001-001)

摘　　要：以大豆天隆1号为材料提取基因组DNA,分别克隆到长2 631 bp的Gm COL1启动子和2 809 bp的Gm COL13启动子。此外,还克隆到Gm COL1和Gm COL13基因组基因长度分别为3 488 bp和2 798 bp,它们分别编码含348个和366个氨基酸的蛋白质。利用Plant CARE分析Gm COL1和Gm COL13的启动子序列发现,它们均含有以下调控元件:生物钟元件(Circadian)、光响应元件(ACEG-box等)、ABA响应元件(ABRE)、干旱诱导元件(MBS)、低温响应元件(LTR)。Prot Param分析表明,Gm COL1和Gm COL13蛋白质的分子量分别为38.47 k D和40.91 k D,并且都是亲水性蛋白。GOR分析显示,Gm COL1和Gm COL13的二级结构含有Alpha helix,Extended strand和Random coil。在线网站PROSITE的分析说明,Gm COL1和Gm COL13有两个Zinc finger B-box和一个CCT domain(CO,CO-LIKE and TOC1 domain)结构域。In the present study,the promoters and genomic genes of Gm COL1 and Gm COL13 were cloned from soybean cultivarTianlong 1. The lengths of Gm COL1 and Gm COL13 promoters were 2 631 bp and 2 809 bp,while their genomic genes were 3 488 bp and 2 798 bp encoding 348 amino acids and 366 amino acids,respectively. Analysis by Plant CARE demonstrated that some important elements in thepromoters of both Gm COL1 and Gm COL13 ：circadian,cis-acting regulatory element involved in light response,cis-acting element involvedin ABA response,MYB binding site involved in drought-induction,and cis-acting element involved in low-temperature response. Prot Paramanalysis showed that Gm COL1 and Gm COL13 proteins were 38.47 k D and 40.91 k D,respectively,and both were hydrophilic. GOR analysisrevealed that their secondary structure included mainly alpha helix,extended strand and random coil. Online PROSITE investigation displayedthat Gm COL1 and Gm COL13 both had two zinc finger B-boxes and one CCT domain.

关 键 词：大豆 GM COL1 GM COL13 基因克隆 生物信息学分析 

分 类 号：Q943.2[生物学—植物学] S565.1[农业科学—作物学]