固相萃取-高效液相色谱-荧光法测定方便面和烤肠中的苯并[a]芘  

Determination of Benzo[a]pyrene in Instant Noodles and Sausage by Solid Phase Extraction-High Performance Liquid Chromatography with Fluorescence Detection

作　　者：童圆[1] 彭西甜[1,2] 龚艳[2] 韩雅荃 吕江潮 冯钰锜[1] 

机构地区：[1]生物医学分析化学教育部重点实验室,武汉大学化学系,湖北武汉430072 [2]湖北省农业科学院农业质量标准与检测技术研究所,湖北武汉430064 [3]长江大学生物工程系,湖北荆州434020

出　　处：《分析科学学报》2017年第4期450-454,共5页Journal of Analytical Science

基　　金：国家自然科学基金(No.21607039);生物医学分析化学教育部重点实验室(武汉大学)开放课题基金(No.ACBM2016004);湖北省农科院竞争性计划(No.2016jzxjh017)

摘　　要：建立了固相萃取-高效液相色谱-荧光法检测方便面和烤肠中苯并[a]芘的方法。采用正己烷作为提取溶剂,经苯并[a]芘专用固相萃取柱HiCapt Benzo富集净化,高效液相色谱-荧光法对样品中苯并[a]芘进行分离分析。苯并[a]芘的质量浓度在0.5~20.0μg/kg范围内与色谱峰面积呈良好的线性关系,相关系数R2为0.9997。方便面和烤肠中苯并[a]芘的加标回收率分别为92.2%~98.3%和95.9%~97.9%,日内和日间相对标准偏差分别为3.34%~5.01%和2.11%~4.07%。与传统方法相比,该方法快速简单、有机溶剂消耗少,在油炸烟熏食品的苯并[a]芘分析中具有较大应用前景。A method for the determination of benzo[a]pyrene in the instant noodles and sausage was established by solid phase extraction-high performance liquid chromatography with fluorescence detection.Using n-hexane as the extraction solvent,the benzo[a]pyrene specialized SPE column（HiCapt Benzo）was used to clean up and enrich analyte.The concentrations of benzo[a]pyrene were linear with chromatographic peak areas in the range of 0.5-20.0μg/kg with the correlation coefficient square of0.9997.The recoveries of benzo[a]pyrene at low,middle and high concentrations in instant noodles and bread sausage were 92.2%-98.3% and 95.9%-97.9%,respectively,and the intra-and inter-day relative standard deviations were 3.34%-5.01%and 2.11%-4.07%,respectively.Compared with the traditional methods,the present method was very quick,easy,and environmental-friendly,demonstrating the great application potential for the analysis of benzo[a]pyrene in smoked and fried food.

关 键 词：苯并[A]芘 固相萃取 方便面 烤肠 高效液相色谱-荧光法 

分 类 号：O657.72[理学—分析化学]