贻贝蛋白-多糖热水同提工艺及抽提物的研究  

Mussel Protein-Polysaccharide Simultaneous Hot Water Extracting Technology and Antioxidant Activity of Extract

作　　者：李玉环[1] 王秀敏[1] 郭晓华 

机构地区：[1]日照职业技术学院海洋工程学院,山东日照276826 [2]山东美佳基团有限公司,山东日照276800

出　　处：《钦州学院学报》2017年第7期5-10,共6页Journal of Qinzhou University

基　　金：山东省科技计划项目:贻贝抗氧化抗高血脂富硒保健食品生产技术研究(2013GGA11027)

摘　　要：用热水同提贻贝蛋白和多糖得到具有抗氧化活性的热水抽提物。贻贝热水抽提工艺条件为:温度70℃,料液比1:20,p H10.0,提取时间3h,得率为31.57%。贻贝热水抽提物多糖含量为69.12%,蛋白质含量为23.61%。抗氧化活性测定表明贻贝热水抽提物具有较高的还原力、自由基清除能力和脂质过氧化抑制作用。DPPH自由基、超氧自由基、羟基自由基和脂质过氧化的半数清除(抑制)浓度SC50分别为3.27mg/m L、4.78mg/m L、5.89mg/m L和2.24mg/m L。The mussel extract with antioxidant activity was obtained by protein-polysaccharide simultaneous hot water extrac- tion. The best extraction condition was as follows ： 70 ℃ , material-water ratio of 1 ： 20, pH 10.0, 3h and the yield reached 31.57%. The mussel hot water extract contained 69.12% polysaccharide and 23.61% protein. The results showed that the ex- tact possessed relatively high reducing power, scavenging activity of free radical and inhibition activity of lipid peroxidation. The SC50 of scavenging DPPH free radical, superoxide radical and hydroxyl radical and inhibition of lipid peroxidation were 3.27mg/mL ,4. 78mg/mL ,5.89mg/mL 和 2.24 mg/mL respectively.

关 键 词：贻贝 热水抽提物 抗氧化活性 

分 类 号：P745[天文地球—海洋生物学]