用BrdU法研究γ射线外照射诱发外周血淋巴细胞HPRT基因突变  

USING BRDU LABELING TO DETECT THE HPRT GENE MUTATION FREQUENCIES INDUCED BY γ RAYS

作　　者：赵涛[1] 崔凤梅[1] 赵经涌[1] 劳勤华[1] 王六一[1] 

机构地区：[1]苏州大学核医学院,苏州215007

出　　处：《辐射研究与辐射工艺学报》2002年第3期187-190,共4页Journal of Radiation Research and Radiation Processing

基　　金：国防科工委 (Y5 5 730 6 2 )资助

摘　　要：用 5 -溴脱氧尿嘧啶 (BrdU)法检测 ,不同剂量γ射线外照射诱发正常人外周血淋巴细胞HPRT基因突变频率 (MFs) ,并研究其剂量效应关系。抽取正常成年人静脉血 ,分成 5组 ,分别用 0 .0— 4 .0Gy的不同剂量γ射线照射 ,全血培养后用BrdU法检测淋巴细胞HPRT基因MFs。结果表明 ,正常人外周血淋巴细胞的MFs随外照射剂量的增加而上升 ,且剂量效应关系符合线性平方模型。研究证明了BrdU法是一种快速、简便、较敏感的检测外照射诱发HPRT基因突变的方法 ,HPRT基因辐射突变可作为生物剂量计。Using BrdU labeling to detect the HPRT gene mutation frequencies(MFs)of a normal adult induced by γ rays of different doses and to analysis the relationship between the HPRT gene MFs and the radiation doses. Blood was drawn via the vein of a normal adult and was equally divided into five groups. The blood was exposed to γ rays with a dose range from 0.0Gy to 4.0Gy respectively. After having cultivated the whole blood, we got the HPRT gene MFs of the lymphocytes. With the increase of the doses, the MFs of the HPRT gene rose accordingly. The result also showed that there is a linear-square relationship between the doses of the γ ray and the MFs of the HPRT gene. According to the result, we may conclude that BrdU labeling method is a quick, simple and relatively sensitive method for detecting the HPRT gene mutations of somatic cells induced by external radiation exposure and HPRT gene mutation may be used as a radiobiological dosimeter.

关 键 词：γ射线外照射 外周血淋巴细胞 基因突变 BrdU法 HPRT基因 突变频率 剂量效应关系 人体 

分 类 号：R331.144[医药卫生—人体生理学] Q754[医药卫生—基础医学]