氧化苦参碱对皮肤鳞状细胞癌Colo-16细胞株增殖及对EGFR表达的影响  

Effects of oxymatrine on the proliferation of cutaneous squamous cell carcinoma Colo-16 cell and the expression of EGFR

作　　者：张青松[1] 陈旭[2] 黄丹[2] 顾恒[2] 

机构地区：[1]常熟市中医院皮肤科,常熟215500 [2]中国医学科学院北京协和医学院皮肤病研究所,南京210042

出　　处：《中国麻风皮肤病杂志》2017年第8期477-480,共4页China Journal of Leprosy and Skin Diseases

基　　金：苏州市科技局立项课题(编号:SYSD2013017);常熟市科技局立项资助课题(编号:CS201319)

摘　　要：目的:明确氧化苦参碱对皮肤鳞状细胞癌(cS CC)Colo-16细胞株增殖及对EGFR表达的影响。方法:体外培养Colo-16细胞株,分为空白对照组、5%葡萄糖溶剂对照组、不同浓度氧化苦参碱(0.025 mg/mL、0.05 mg/mL、0.1 mg/mL、0.5 mg/mL、1 mg/mL、2 mg/mL)组。MTT法测定各组Colo细胞株增殖情况,Western blot印迹法测定各组表皮生长因子受体(EGFR)及磷酸化表皮生长因子受体(pE GFR)的表达。结果:氧化苦参碱的各浓度组在24 h、48 h、72 h OD值均低于空白对照组及溶剂对照组,差异具有统计学意义(P<0.05)。氧化苦参碱各浓度组的OD值无显著差异(P>0.05)。不同浓度组与空白对照组相比及各不同浓度组之间,EGFR及磷酸化EGFR的表达差异均无统计学意义(P>0.05)。结论:氧化苦参碱在较低的浓度即有抑制Colo-16细胞株增殖的作用,而提高浓度不能增强抑制作用。氧化苦参碱并非通过抑制EGFR的表达来影响Colo-16细胞的增殖。Objective： To determine the effects of oxymatrine on the proliferation of cutaneous squamous cell carcinoma Colo-16 cell and the expression level of EGFR. [WT5＂HZ]Methods： The Colo-16 cells were cultured,and then were divided into the blank control group,5% glucose solvents control group and different concentration of oxymatrine groups（ 0.025 mg/mL,0.05 mg/mL,0.1 mg/mL,0.5 mg/mL,1 mg/mL and 2mg/mL）. The proliferative activity of Colo-16 cell and the expression level of EGFR and phosphorylate EGFR were detected by MTT and Western blot respectively. Results： The optical density in the different concentration of oxymatrine were lower than those in the blank control group and 5% glucose solvents control group at 24 h,48 h and 72 h,with significant differences（ P0.05）. However,there was no significant difference of optical density between the different concentration of oxymatrine groups（ P 0. 05）. There was no significant difference in the levels of EGFR and phosphorylate EGFR between all the groups（ P 0. 05）. Conclusion：Oxymatrine can inhibit the proliferative activity of Colo-16 cell at lower concentration,in a non-concentration-dependent manner. Therefore,the effect of oxymatrine on the proliferative activity is not through inhibition of the expression of EGFR.

关 键 词：氧化苦参碱 皮肤鳞状细胞癌 增殖 EGFR 

分 类 号：R739.5[医药卫生—肿瘤]