荧光素与β-环糊精的包合作用  

The Clathration between Fluorescein and β-cyclodextrin

作　　者：黎泓波[1,2] 唐永琼 王素琴[1,2] 王丹[3] 王兴明[3] 

机构地区：[1]功能有机小分子教育部重点实验室,江西南昌330022 [2]江西师范大学化学化工学院,江西南昌330022 [3]西南科技大学材料科学与工程学院,四川绵阳621010

出　　处：《湖南生态科学学报》2017年第2期25-30,共6页Journal of Hunan Ecological Science

基　　金：国家自然科学基金资助项目(编号:21565015;21663014);江西省教育厅基金资助项目(编号:GJJ150363);湖南大学化学生物与计量学国家重点实验室开放基金(编号:Z2015022);功能有机小分子教育部重点实验室开放基金(编号:KLFS-KF-201425)

摘　　要：采用UV光谱法、荧光光谱法、双倒数法,在pH=7.40的缓冲溶液中系统研究小分子荧光素与β-环糊精的包合作用.摩尔比法确定小分子荧光素与β-环糊精的包合比n_(β-CD):n_(FL)=1∶1,表观摩尔吸光系数ε=6.30×10~4L/(mol·cm).双倒数法确定结合常数K~Θ 18℃=7.21×10~5L/mol.热力学分析方法计算得到:(1)Δ_rH_m~Θ=7.93×10~4J/mol,Δ_rH_m~Θ为正值,说明包合作用吸热;(2)Δ_rG_m~Θ 291.15 K=-3.27×10~4J/mol,推测β-环糊精与荧光素的包合作用有自发进行的可能;(3)Δ_rS_m~Θ 291.15 K=384.68 J/(mol·K),由此可知,β-环糊精与荧光素的相互作用为熵所驱动.并用傅里叶变换红外光谱仪和X射线衍射仪对包合物进行了表征.The clathration between the small-molecule fluorescence and β-cyclodextrin in physiological pH environment （pH7.40） was studied with UV spectra,fluorescence spectra and double reciprocal method.The result showed that fluorescence and β-cyclodextrin formed a inclusion complex with binding ratio nβ-D ：nFE = 1 ： 1, and the molor absorptivity of ε=6.30×10~4L/（mol·cm） by molar ratio method. The binding constant of KΘ 18℃=7.21×10~5L/mol was confirmed by double reciprocal method.The followings were calculated by the thermodynamic analysis method： （ 1 ） Δ_rH_m~Θ=7.93×10~4J/mol, in which ΔrHm~Θ is positive, indicating an endothermic reaction ; suggesting that the interaction of β-cyclodextrin withfluorescein spontaneously; （3）Δ_rS_m~Θ 291.15 K=384.68 J/（mol·K） , from which entropy worked as driven force in this action. β-eyclodextrin-fluorescein was synthesized and characterized by fourier transform infrared spectrometer and X-ray diffractometer.6figs. ,20refs.

关 键 词：荧光素 Β-环糊精 包合物 包合作用 

分 类 号：TB34[一般工业技术—材料科学与工程] O613.7[理学—无机化学]