海洋小杂鱼制备亚铁结合肽的酶促水解条件优化  

Optimization of Ferrous Binding Peptides from Marine Low-value Fish by Enzymatic Hydrolysis

作　　者：杨青伟 黄光荣 

机构地区：[1]中国海洋大学海洋生命学院,青岛266003 [2]中国计量大学生命科学学院,杭州310018 [3]浙江省海洋食品品质及危害物控制技术重点实验室,杭州310018

出　　处：《食品工业》2017年第9期6-10,共5页The Food Industry

基　　金：浙江省科技计划项目(2016C32064)

摘　　要：以海燕鱼和小银鱼等海洋小杂鱼为原料,用商品蛋白酶水解,制备具有较高亚铁结合能力的小肽水解液。结果表明,在四种商品蛋白酶中风味蛋白酶水解的酶促水解液具有更高的亚铁结合能力。在单因素试验确定影响风味蛋白酶水解因素的取值范围基础上,利用Box-Benhnken中心组合设计,采用响应面法对风味蛋白酶水解海洋小杂鱼蛋白质制备亚铁结合肽的酶促水解工艺进行了优化,得到最佳水解条件为:水解时间125 min、料液比值3.0(g/100 mL)、酶添加量14.8 U·mg^(-1)蛋白。在最佳水解条件下,模型预测亚铁结合能力和水解度分别为74.4%和30.5%,三组验证试验值分别为73.5%和31.5%,与预测值接近。建立的响应面模型可用于实际酶促水解过程预测,为制备亚铁结合肽提供一定理论基础。The enzymatic hydrolysate with high ferrous binding ability was prepared from marine low-value fish （swallow fish and grunion） by commercial proteases. The results improved that flavourzyme was the best choice for producing high ferrous binding hydrolysate. Based on the single-factor experiments, Box-Behnken central composite design in response surface methodology method （RSM） was used to optimize the enzymatic hydrolysis conditions for preparation of ferrous binding peptides from marine low-value fish. The best hydrolysis conditions by RSM were as following： hydrolysis time of 125 min, solid-liquid ratio of 3.0 （g/100 mL） enzyme substrate ratio of 14.8 U-tugl-protein. The estimated value of ferrous binding ability and degree of hydrolysis at these optimized hydrolysis conditions were 74.4% and 30.5% respectively, which were very closed to the three verified experiments values of 73.5% and 31.5%. This predicated model could be used to predict the experiment results well and had certain theoretical basis for nret）aration of ferrous binding Deotides.

关 键 词：海洋小杂鱼 亚铁结合肽 酶促水解 响应面法 

分 类 号：TQ914.2[化学工程]