基于EST-SSR标记与果实品质性状的贵州野生刺梨核心种质构建  

Construction of Core Collection in Wild Rosa roxburghii from Guizhou Province Using EST-SSR Markers and Fruits Quality Traits

作　　者：鲁敏[1] 鄢秀芹 白静[1] 张怀山[1] 王道平[2] 安华明[1] 

机构地区：[1]贵州大学农学院/贵州省果树工程技术研究中心,贵阳550025 [2]贵州省中国科学院天然产物化学重点实验室,贵阳550002

出　　处：《园艺学报》2017年第8期1486-1495,共10页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31660558);贵州省高层次创新型人才培养计划项目(黔科合人才20164016);贵州省科技计划项目(黔科合SY字20153026-1)

摘　　要：采用10对EST-SSR引物和9个果实品质性状指标对收集的220份野生刺梨(Rosa roxburghii Tratt)资源的遗传多样性进行分析,结果表明:10对EST-SSR引物共扩增出多态性条带27条,多态百分率为76.67%;9个品质性状中平均单果质量、可滴定酸、固酸比、维生素C和类黄酮含量的变异系数达到20%以上;说明该220份贵州野生刺梨种质资源遗传变异丰富,遗传多样性分布范围较广,适于进行核心种质的构建研究。进一步将9个品质性状指标进行6级分类,并处理为9个品质标记共产生54条多态带,与EST-SSR标记一起,采用Nei’s遗传距离进行UPGMA聚类,同时采用位点优先取样策略进行核心种质构建,以表型保留比例、均值差异百分率、方差差异百分率、极差符合率、变异系数变化率、多态性位点百分率、Nei’s基因多样性指数、Shannon’s信息指数等对核心种质进行评价,并利用主坐标分析法对核心种质进行确认。结果表明:构建的32份核心种质在分子水平,表型水平及地区分布上都能够代表原种质的遗传多样性。Genetic diversity of 220 wild Rosa roxburghii accessions was investigated using 10 EST-SSRs and quality traits. A total of 33 alleles were amplified by 10 pairs of EST-SSR primers, in which polymorphic alleles was 27. The polymorphic percentage was 76.67%. The coefficients of variation of 9 quality traits were all greater than 10%, in which the average fruit weight, titratable acid, total soluble solid/titratable acid, vitamin C and flavonoids content were above 20%. The 220 wild R. roxburghii germplasm resources in Guizhou were rich in genetic diversity in a wide range, suitable for the construction of the core collection. Nine quality traits were each further classified into 6 grades, treated as that 54 polymorphic alleles, were produced by 9 quality markers, together with EST-SSR markers, were performed for UPGMA clustering using Nei＇s genetic distance. At the same time, the core collection was constructed by using the allele preferred sampling strategy. The ratio of phenotype retained, mean difference percentage, variance difference percentage, coincidence rate of range, changeable rate of coefficient of variation, percentage of polymorphic loci, Nei＇s gene diversity index and Shannon＇s information index were used to evaluate core collection. Principal coordinate analysis method was used to confirm core collection. Results showed that the 32 core collections can represent the genetic diversity of the original germplasm at the molecular level, the phenotypic level and the regional distribution.

关 键 词：刺梨 EST-SSR 果实品质 遗传多样性 核心种质 

分 类 号：S661.9[农业科学—果树学]