生物催化法制备手性酮基布洛芬的研究进展  

Progress in biosynthesis of chiral ketoprofen

作　　者：胡琦蔚 张俊伟 王远山 

机构地区：[1]浙江工业大学生物工程学院,浙江杭州310014 [2]杭州安诺过滤器材有限公司,浙江杭州311404

出　　处：《发酵科技通讯》2017年第3期153-157,共5页Bulletin of Fermentation Science and Technology

基　　金：浙江省公益技术研究社会发展项目(2014C33223)

摘　　要：酮基布洛芬是一种重要的消炎镇痛药,广泛应用于风湿、类风湿性关节炎、脊髓炎和痛风等疾病的治疗,成为处方量最大的药物之一.市售的酮基布洛芬以外消旋体为主,(S)-酮基布洛芬为其活性对映体,(R)-酮基布洛芬的存在会加重肝脏的代谢负担.目前手性酮基布洛芬主要通过化学合成法生产,与传统的化学法相比,生物催化法具有反应条件温和、对环境友好等优点,成为近年来研究的热点.生物催化法主要利用脂肪酶、酯酶和腈水合酶/酰胺酶双酶体系等催化制备手性酮基布洛芬.同时对生物催化法制备手性酮基布洛芬的研究进展进行了综述.Ketoprofen is one of the most prominent non-steroidal anti-inflammatory drugs,which was widely used in rheumatism,rheumatoid arthritis,myelitis,gout and other diseases.Most of the commercially available ketoprofen drugs are racemic.However,the（S）-enantiomer has therapeutic activity against inflammation and pain,while the existence of（R）-enantiomer resulted in increased physiological side effects and toxicity.Chiral ketoprofen is currently produced by chemical synthesis.Compared to chemical methods,biosynthesis of ketoprofen is preferable because of its mild reaction conditions and eco-friendliness.In this review,the progress in biosynthesis of chiral ketoprofen with lipase,esterase,and nitrile hydratase/amidase bienzyme system as biocatalysts was summarized.

关 键 词：生物催化 酮基布洛芬 脂肪酶 酯酶 

分 类 号：Q814[生物学—生物工程]