纤维素酶辅助提取花椒挥发油工艺及其抗氧化作用的研究  

Study on Process of Cellulase Assisted Extraction of Volatile Oil from Zanthoxylum bungeanum Maxim. and Its Antioxidation Effect

作　　者：杜兵兵[1] 

机构地区：[1]漯河医学高等专科学校,河南漯河462002

出　　处：《中国调味品》2017年第9期55-57,共3页China Condiment

摘　　要：采用纤维素酶辅助提取花椒挥发油,在单因素试验基础上,设计正交试验。分析各个因素的显著性,得出纤维素酶辅助提取花椒挥发油的最佳工艺条件:纤维素酶的添加量为2%,预处理温度为40℃,预处理时间为2h,pH为4.6。在此条件下,花椒挥发油得率为11.58%。而且花椒挥发油对DPPH自由基具有一定的清除能力,EC50为9.611mg/mL。Cellulase is used for assisted extraction of volatile oil from Zanthoxylum bungeanurn Maxim. and orthogonal experiment is designed on the basis of single factor experiment. The optimum conditions are as follows： the additive amount of cellulase is 2%, the pretreatment temperature is 40 %, the pretreatment time is 2 h and the pH value is 4. 6. Under such conditions, the yield of volatile oil is 11.58%, and the volatile oil of Zanthoxylum bungeanurn Maxim. has certain scavenging ability to DPPH free radical, EC50 is 9. 611 mg/mL.

关 键 词：花椒 挥发油 纤维素酶 提取工艺 抗氧化 

分 类 号：TS264.2[轻工技术与工程—发酵工程]