Suppression of fibrosis in human pterygium fibroblasts by butyrate and phenylbutyrate  

作　　者：Yuka Koga Noriaki Maeshige Hiroto Tabuchi Mikiko Uemura Michiko Aoyama-Ishikawa Makoto Miyoshi Chikako Katakami Makoto Usami 

机构地区：[1]Division of Nutrition and Metabolism,Department ofBiophysics,Kobe University Graduate School of Health Sciences,Tomogaoka 7-10-2,Suma-ku,Kobe,Japan [2]Department of Rehabilitation Science,Kobe University Graduate School of Health Sciences,Tomogaoka 7-10-2,Suma-ku,Kobe,Japan [3]Department of Ophthalmology,Saneikai Tsukazaki Hospital,Waku 68-1,Aboshi-ku,Himeji,Japan [4]Department of Nutrition,Kobe University Hospital,Kobe University School of Medicine,Kusunoki-cho 7-5-2,Chuo-ku,Kobe,Japan

出　　处：《International Journal of Ophthalmology(English edition)》2017年第9期1337-1343,共7页国际眼科杂志（英文版）

基　　金：Supported by JSPS KAKENHI(No.23592648)

摘　　要：AIM：To evaluate the antifibrogenic effects of butyrate or phenylbutyrate,a chemical derivative of butyrate,in human pterygium fibroblasts.METHODS：Human pterygium fibroblasts obtained from patient pterygium tissue were treated with butyrate or phenylbutyrate for 48h.Expression ofα-smooth muscle actin,collagen I,collagen III and matrix metalloproteinase-1m RNA was measured by quantitative real-time reverse transcription polymerase chain reaction,and acetylated histone was evaluated by Western blotting.RESULTS：Butyrate inhibitedα-smooth muscle actin,type III collagen and matrix metalloproteinase-1 expressions,and phenylbutyrate inhibited types I and III collagen and matrix metalloproteinase-1 expressions without changing cell viability as well as both of these increased histone acetylation.These results suggested that butyrate and phenylbutyrate suppress fibrosis through a mechanism involving histone deacetylase inhibitor.CONCLUSION：This indicates that butyrate or phenylbutyrate have antifibrogenic effects in human pterygium fibroblasts and could be novel types of prophylactic and/or therapeutic drugs for pterygium,especially phenylbutyrate,which does not have the unpleasant smell associated with butyrate.AIM：To evaluate the antifibrogenic effects of butyrate or phenylbutyrate,a chemical derivative of butyrate,in human pterygium fibroblasts.METHODS：Human pterygium fibroblasts obtained from patient pterygium tissue were treated with butyrate or phenylbutyrate for 48h.Expression ofα-smooth muscle actin,collagen I,collagen III and matrix metalloproteinase-1m RNA was measured by quantitative real-time reverse transcription polymerase chain reaction,and acetylated histone was evaluated by Western blotting.RESULTS：Butyrate inhibitedα-smooth muscle actin,type III collagen and matrix metalloproteinase-1 expressions,and phenylbutyrate inhibited types I and III collagen and matrix metalloproteinase-1 expressions without changing cell viability as well as both of these increased histone acetylation.These results suggested that butyrate and phenylbutyrate suppress fibrosis through a mechanism involving histone deacetylase inhibitor.CONCLUSION：This indicates that butyrate or phenylbutyrate have antifibrogenic effects in human pterygium fibroblasts and could be novel types of prophylactic and/or therapeutic drugs for pterygium,especially phenylbutyrate,which does not have the unpleasant smell associated with butyrate.

关 键 词：BUTYRATE phenylbutymte PTERYGIUM FIBROBLASTS antifibrogenic effect 

分 类 号：R777.33[医药卫生—眼科]