2012—2014年中国婴儿配方食品和谷基辅食类食品及临床腹泻病例来源的克罗诺杆菌种水平鉴定研究  

Study on species identification of Cronobacter isolated from infant formula foods and cereal based foods and clinical diarrhea cases in 2012-2014 in China

作　　者：甘辛 王伟 胡豫杰 李凤琴 毛旭 徐进 

机构地区：[1]国家食品安全风险评估中心卫生部食品安全风险评估重点实验室,北京100021

出　　处：《卫生研究》2017年第5期695-698,704,共5页Journal of Hygiene Research

基　　金：北京市自然科学基金(No.S150004)

摘　　要：目的建立一种基于细菌RNA聚合酶β亚基编码基因(rpo B)的克罗诺杆菌种鉴定PCR方法,对不同来源的克罗诺杆菌进行种水平鉴定。方法通过rpo B基因设计针对克罗诺杆菌7个种的引物,以9株参考菌株作为参照,对261株2012—2014年收集的我国婴儿配方食品和谷基辅食类食品及临床腹泻病例来源的克罗诺杆菌进行种水平鉴定。结果 9株参考菌株PCR结果与对应大小一致,261株克罗诺杆菌中的179株为阪崎克罗诺杆菌,56株为丙二酸盐克罗诺杆菌,13株为尤尼沃斯克罗诺杆菌,11株为都柏林克罗诺杆菌,2株为苏黎世克罗诺杆菌,分别占总菌株数的68.58%、21.46%、4.98%、4.21%和0.77%。结论建立的克罗诺杆菌PCR法种水平鉴定具有特异、便捷、灵敏等优势,可为我国食品安全克罗诺杆菌的风险监测和控制提供技术支持。Objective To develop a Cronobacter spieces identification PCR method based on RNA polymerase beta subunit（ rpo B） gene and identify species of Cronobacter isolated from different sources. Methods A total of 7 primer pairs based on the rpo B were design,and 9 standard strains were set as reference strains to identify species of261 strains isolated from infant formula foods,cereal based foods and clinical diarrhea cases in 2012-2014 in China. Results The molecular weight of 9 standard strains were as same as expect,179 of 216 Cronobacter isolates were C. sakazakii,56 were C.malonaticus,13 were C. universalis,11 were C. dublinensis,2 were C. turicensis,accounted for 68. 58%,21. 46%,4. 98%,4. 21% and 0. 77% of the total isolates,respectively. Conclusion The Cronobacter species identification PCR system is specific,convenient,sensitive. This system can provide technical support for Cronobacter risk surveillance and control in China.

关 键 词：克罗诺杆菌 PCR 食品安全 婴幼儿食品 

分 类 号：R155.51[医药卫生—营养与食品卫生学] R378.2[医药卫生—公共卫生与预防医学]