基于纳米金与适配体的赭曲霉毒素A检测方法研究  

Study on detection method for ochratoxin A based on gold nanoparticle and aptamer

作　　者：吕蕾[1] 赵阳阳[2,3] 刘仁杰[3] 金永东[2] 郭志军[1] 

机构地区：[1]延边大学农学院,吉林延吉133002 [2]中国科学院长春应用化学研究所,吉林长春130022 [3]吉林农业大学生命科学学院,吉林长春130118

出　　处：《食品与机械》2017年第7期56-60,共5页Food and Machinery

基　　金：吉林省科技厅青年科研基金项目(编号:20160520047JH);吉林省教育厅科学技术研究项目(编号:吉教科合字[2016]第252号);国家自然科学基金项目(编号:31360384;31460423)

摘　　要：以赭曲霉毒素A适配体作为识别分子荧光素发射的荧光作为检测信号、纳米金作为荧光信号淬灭剂,建立一种高灵敏度的赭曲霉毒素A荧光检测方法。结果表明:该检测体系检测限达到10nmol/L,在25~1 000nmol/L浓度范围具有线性关系,相关系数为0.991,具有较好的实用性。该荧光检测法同传统方法相比,提高了检测效率,为更好地进行赭曲霉毒素A检测控制提供了技术支持。A high sensitivity fluorescence detection method for ochratoxin A（OTA）based on OTA aptamers and gold nanoparticle（AuNPs）was established,which employed OTA aptamers as recognition element and AuNPs to quench the fluorescence emission from fluorescein.The results indicated that the detection limit is 10nmol/L and a linear relationship between the fluorescent intensity ratio and the logarithm of OTA concentration was plotted in the range of 25~1 000nmol/L（R^2=0.991）.The detection method showed high feasibility and reliability.Compared with traditional method,this method greatly improved the detection efficiency and provided technical support for more effective identification of OTA.

关 键 词：纳米金 适配体 赭曲霉毒素A 生物传感器 

分 类 号：O657.3[理学—分析化学] TS207.5[理学—化学]