微切助互作技术提取湘玉竹总黄酮工艺研究  

Extraction of total flavonoids by press-shear assisted interaction technology from Polygonatum odoratum

作　　者：李化强 吴菲菲[1,2,3,4] 龙艳珍 赵良忠[1,2] 徐永平 李淑英 LI Hua-Qiang WU Fei-Fei LONG Yan-Zhen ZHAO Liang-Zhong XU Yong-Ping LI Shu-Ying(College of Food and Chemistry Engineering, Shaoyang University, Shaoyang 422000, China Hunan Provincial Engineering and Technology Research Center for Fruit and Vegetable Clean Processing, Shaoyang 422000, China School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China Postdoctoral Research Stations of Dalian SEM Bio-Engineering Technology Co., Ltd., Dalian 116620, China)

机构地区：[1]邵阳学院食品与化学工程学院,邵阳422000 [2]湖南省果蔬清洁加工工程技术研究中心,邵阳422000 [3]大连理工大学生命科学与生物技术学院,大连116024 [4]大连赛姆生物工程技术有限公司博士后科研工作站,大连116620

出　　处：《食品安全质量检测学报》2017年第8期3056-3061,共6页Journal of Food Safety and Quality

基　　金：国家科技部863计划项目(2013AA102805-03);湖南省果蔬清洁加工工程技术研究中心项目(2015TP2022);湖南省自科基金青年项目(2017JJ3284);湖南省教育厅优秀青年项目(17B241);湖南省教育厅一般项目(17C1433)~~

摘　　要：目的优化微切助互作技术提取湘玉竹总黄酮的工艺条件。方法以磺丁基醚-β-环糊精为助剂,湘玉竹为原料,总黄酮提取率为指标,采用单因素与正交结合试验确定最佳提取工艺。结果最佳提取工艺为:磺丁基醚-β-环糊精添加量3%(m:m),提取时间60min,水浴温度50℃,丙酮浓度70%(V:V)。该条件下总黄酮提取率为1.72%,与热回流法相比提取率提高48.28%,且提取总时长由122min缩短到82min,以70%丙酮作提取溶剂,减少了有机溶剂的使用量。结论该技术能够高效、低成本地提取湘玉竹总黄酮,具有潜在的良好应用前景。Objective To optimize process conditions of total flavonoids extraction from Polygonatum odoratum by press-shear assisted interaction （PAI） technology. Methods The optimum parameters of total flavonoids were determined using sulfobutyl ether-β-cyclodextrin as additive, Polygonatum odoratum as materials, and total flavonoids yield as an index in single factor analysis and orthogonal test. Results The optimum process conditions of total flavonoids were as follows： sulfobutyl ether-β-cyclodextrin was 3% （m：m）, extraction time was 60 min, bath temperature was 50 ℃ and acetone concentration was 70% （V：V）. The highest total flavonoids yield of Polygonatum odoratum was up to 1.72% which increased by 48.28% than that of hot reflux, the total time was shortened from 122 min to 82 min, and the amount of acetone was reduced. Conclusion The PAI technology exerts an efficient and low-cost method for extraction of total flavonoids from Polygonatum odoratum with a potential application prospect.

关 键 词：微切助互作技术 湘玉竹 总黄酮 磺丁基醚-Β-环糊精 

分 类 号：TQ28[化学工程—有机化工]