气相色谱-质谱法同时测定肉制品中的12种多环芳烃  

Simultaneous determination of 12 polycyclic aromatic hydrocarbons in meat products by gas chromatography-mass spectrometry

作　　者：曾莉[1] 张炤[1] 刘丹[1] 王晓鹏[1] 

机构地区：[1]宜宾市疾病预防控制中心,四川宜宾644000

出　　处：《中国卫生检验杂志》2017年第17期2486-2488,2504,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的建立肉制品中12种多环芳烃化合物的气相色谱-质谱(GC/MS)同时测定方法。方法样品经搅碎混匀后,称取一定量经正己烷-丙酮溶液(体积比为1∶1)超声波提取,提取液经中性氧化铝小柱净化,以正己烷-二氯甲烷溶液(体积比为1∶1)洗脱后浓缩,正己烷定容后,用气相色谱-质谱法分离测定。结果本法在0.1μg/ml^5.0μg/ml时有良好的线性关系,相关系数为0.996 7~0.999 9,仪器检出限为0.04 ng/ml^45 ng/ml,除二苯并(a,h)蒽的定量限为49μg/kg外,其余PAHs的定量限为0.04μg/kg^3.9μg/kg。结论利用超声提取、中性氧化铝小柱净化、气相色谱法分离、质谱法检测建立的肉制品中12种多环芳烃化合物的同时测定方法具有简单、易操作、准确度和重现性好的特点。Objective To develop a method for simultaneous determination of 12 polycyclic aromatic hydrocarbons（ PAHs） in meat products by gas chromatography-mass spectrometry（ GC/MS）. Methods The sample was minced and mixed,and a portion of the sample was ultrasonically extracted with n-hexane-acetone（ 1∶1,V/V） and cleaned-up with a neutral alumina column and then eluted with n-hexane-dichloromethane（ 1∶1,V/V） from the column and concentrated. The residue was dissolved with n-hexane and determined by GC/MS. Results Within the range of 0. 1 μg/ml-5. 0 μg/ml,the linearity of this method was good with the correlation coefficients of 0. 996 7-0. 999 9. The instrument detection limits were within 0. 04 ng/ml-45 ng/ml,and the quantification limits,except dibenzo（ a,h） anthracene（ 49 μg/kg） were both in the range of 0. 04 μg/kg-3. 9 μg/kg. Conclusion The method for 12 PAHs determination in meat products combined with the ultrasonic extraction,neutral alumina column purification and gas chromatography-mass spectrometry is simple,easy to operate,accurate and reproducibl.

关 键 词：气相色谱-质谱法 多环芳烃化合物 肉制品 

分 类 号：R155.5[医药卫生—营养与食品卫生学]