利用反相高效液相法测定马乳中的乳清蛋白  

Determination of whey proteins in mare milk by reversed-phase high-performance liquid chromatography

作　　者：李敏婧 杨洁[1] 杨迎春 王红娟[1] 吕岳文 申彤[1] 刘军[1] 阿里木江.阿布都拉 

机构地区：[1]新疆大学生命科与技术学院,新疆乌鲁木齐830049

出　　处：《食品与发酵工业》2017年第9期209-214,共6页Food and Fermentation Industries

基　　金：国家自然科学基金(31471645)

摘　　要：乳清蛋白是马乳中主要的蛋白质,不同物种间蛋白质组成存在差异。该研究利用反相高效液相色谱(RP-HPLC)对马乳清中的蛋白进行分离和定量分析,利用基质辅助激光解吸电离飞行时间质谱(MALDI-TOFMS)对主要分离组分进行鉴定。结果表明,马乳乳清蛋白中主要组分为LYS,α-lg和β-lg。利用建立的色谱分析方法对乳清蛋白定量测定结果显示,LYS,α-lg和β-lg在(0.1~1.0)(R^2=0.999 3)、(0.2~0.8)(R^2=0.996 9)和(0.2~1.0)g/L(R^2=0.990 3)线性关系良好。该方法的稳定性、精密度、重现性的相对标准偏差(RSD)均小于5%,加标回收率分别为:88.05%~96.26%,86.03%~95.70%和91.79%~100.97%,能够满足实际检测的需要。测得马乳乳清中的LYS,α-lg和β-lg,含量为别为(3.38±0.32)、(2.64±0.37)及(0.52±0.06)g/L。Whey protein are the main protein in mare milk, but are different in protein composition among different species. In this paper, reserved-phase high-performance liquid chromatography （RP-HPLC） was used to isolate and quantify proteins in mare whey, the main components were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF-MS）. The result showed that LYS, α-lg and β-lg are the primary components in mare whey proteins. The linear relation of LYS , α-lg and β-lg are in the range of 0.1 - 1.0 （ R^2 = 0. 999 3 ） 0.2 - 0.8 （R^2 = 0. 996 9） and 0.2 - 1.0 μg/L（R^2 = 0. 990 3）, respectively. The relative standard deviations （RSDs） value of precision and reproducibility tests were all less than 5% , and the spike recovery was in the range of 88.05% - 96.26% , 86.03% - 95.70% and 91.79% - 100.97% , respeetively. The concentration of LYS, α-lg and β-lg in mare milk were （0.52±0.06） ,（3.38±0.32） and （2.64 ＋0.37）μg/L, respectively.

关 键 词：反相高效液相色谱法 马乳 溶菌酶 Α-乳白蛋白 Β-乳球蛋白 

分 类 号：O657.72[理学—分析化学] TS252.2[理学—化学]