鲁氏酵母胞内海藻糖积累过程的代谢特征分析  

Metabolic Characteristics of Intracellular Trehalose Accumulation in Zygosaccharomyces rouxii

作　　者：刘翠翠[1] 胡梦蝶 王志[1] 代俊[1] 姚娟[2] 李沛[2] 李志军[2] 陈雄[1] 李欣[1] LIU Cui-cui HU Meng-die WANG Zhi DAI Jun YAO Juan LI Pei LI Zhi-jun CHEN Xiong LI Xin(Key Labortory of Fermentation Engineering（ Ministry of Education）, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei University of Technology,Wuhan 430068, China Hubei Angel Yeast Limited by Share Ltd, Yichang 443003, China)

机构地区：[1]教育部发酵工程重点实验室湖北省工业发酵协同创新中心,湖北工业大学,武汉430068 [2]湖北安琪酵母股份有限公司,宜昌443003

出　　处：《中国生物工程杂志》2017年第9期41-47,共7页China Biotechnology

基　　金：湖北省科技厅重大专项基金(2015ACA052);湖北省教育厅基金(6101-12131)资助项目

摘　　要：海藻糖合成是微生物对抗环境逆境的一种重要途径。研究10L发酵罐中的分批、分批补料及分批补料控温三种不同的海藻糖发酵调控策略下酱油风味形成微生物鲁氏酵母CCTCC M2013310的代谢特征。色谱结果表明,乳酸、丙酮酸和α-酮戊二酸受到不同发酵调控模式的显著影响,但谷氨酸和谷氨酰胺总含量在三种发酵调控模式间却无显著差异。这些结果表明,细胞还原力平衡途径和碳氮调控代谢均对胞内海藻糖的积累产生影响。研究结果为鲁氏酵母CCTCC M2013310的高浓度内源性海藻糖细胞代谢工程改造提供了新思路。Trehalose synthesis is an important pathway to protect cell against environmental stress. The metabolic characteristics of Zygosaccharomyces rouxii CCTCC M2013310 under three different trehalose fermentation control strategies included batch,fed-batch and fed-batch conbined control temperature in 10 L fermentation tank are studied. The results from chromatographic analysis show that lactic acid,pyruvate andα-ketoglutaric acid are significantly affected by different fermentation modes. However,there is no significant difference between the total content of glutamic acid and glutamine in the three fermentation control process.These results show that the accumulation of intracellular trehalose is effected by the cell reduction force balance pathway and the metabolic regulation of carbon and nitrogen metabolism. The results provid a new idea for the metabolic engineering of Z. rouxii CCTCC M2013310 to make the high concentration of endogenous trehalose yeast cell.

关 键 词：鲁氏酵母 海藻糖 发酵调控 氨基酸 有机酸 

分 类 号：Q493.4[生物学—生理学]