胡桃楸种仁壳多糖的分子修饰研究  

Molecular modification of polysaccharides from Juglans mandshurica seed shells

作　　者：程晓强[1] 申鹏飞 李胜男[1] 张金堂 徐红艳[1] CHENG Xiao-qiang SHEN Peng-fei LI Sheng-nan ZHANG Jin-tang XU Hong-yan(Agricultural College of Yanbian University, Yanji 133002 Bureau of Science and Technology in Helong City, Helong 133500)

机构地区：[1]延边大学农学院,延吉133002 [2]和龙市科学技术局,和龙133500

出　　处：《食品科技》2017年第9期196-201,共6页Food Science and Technology

基　　金：吉林省教育厅"十二五"科学技术研究项目(吉教科合字[2015]第41号)

摘　　要：研究胡桃楸种仁壳多糖的分子修饰方法。通过正交试验优化种仁壳多糖羧甲基化和硫酸酯化条件,采用红外光谱法定性分析分子修饰的种仁壳多糖,并比较修饰前后多糖的抗氧化作用。结果表明,采用优化方法进行种仁壳多糖的分子修饰后,多糖分子的主要结构仍保存良好,且分别具有羧甲基化和硫酸酯化特征吸收峰;分子修饰多糖对ABTS的清除作用均高于未修饰多糖,羧甲基化多糖强于硫酸酯化多糖。表明种仁壳多糖分子修饰成功,分子修饰后的种仁壳多糖抗氧化性得到提高,且羧甲基化的分子修饰方法取得了更好的效果。Molecular modification methods of polysaccharides from Juglans mandshurica seed shell were studied. The technologies of carboxymethylation and sulfation of seed shell polysaccharides was optimized by orthogonal test. The qualitative analysis of molecular modification polysaccharides of seed shell was carried out using infrared spectroscopy. And antioxidant ef fects of molecular modification polysaccharides were compared with unmodified polysaccharide. The results showed that the main molecule structure of polysaccharides were well maintained after molecular modification using optimization method, and the characteristic absorption peak of carboxymethlation and sulfation were appeared respectively. The scavenging effect of modified polysaccharides on ABTS free radicals was stronger than unmodified polysaccharides, and the scavenging activity of carboxymethylation polysaccharides was stronger than that of sulfation polysaccharides. The molecular modifications of polysaccharides of seed shell were succeeded. Their antioxidant effects were enhanced by molecular modification. And the better results were obtained by carboxymethylation method of molecular modification.

关 键 词：胡桃楸 种仁壳 多糖 羧甲基化 硫酸酯化 

分 类 号：TS201.2[轻工技术与工程—食品科学]