金鲳鱼鱼皮酶溶性胶原蛋白的提取工艺优化及基本特性  

Optimization of extraction process and basic properties for pepsin-soluble collagen from Golden pompano skin

作　　者：廖伟 夏光华[1] 王佳媚[1] 杨晋坤 李永成[1] 申铉日[1] 

机构地区：[1]海南大学食品学院,海南海口570228

出　　处：《食品工业科技》2017年第19期142-147,共6页Science and Technology of Food Industry

基　　金：海南省自然基金(317038);海南省重点研发计划(ZDYF2017104);海南大学科研启动基金资助项目(kyqd1662)

摘　　要：为提高金鲳鱼加工副产物——鱼皮的利用率,本研究在前期单因素实验的基础上,以加酶量、液料比和提取时间三个单因素建立模型,采用响应面法Box-Behnken设计实验,优化了胃蛋白酶法提取金鲳鱼鱼皮酶溶性胶原蛋白(PSC)的提取工艺,并与酸溶性胶原蛋白(ASC)进行聚丙烯酰胺凝胶电泳、傅里叶变换红外光谱和差示扫描量热仪等基本理化特性的对比研究。结果表明,金鲳鱼鱼皮胶原蛋白的最佳提取工艺为:加酶量1.5%、液料比23∶1 m L/g、酶解时间27.5 h。此条件下通过实验验证得出,金鲳鱼鱼皮胶原蛋白实际提取率为64.68%,与响应面模型理论预测值65.17%基本一致;此外,本实验提取的PSC有较高热变性温度(30.44℃),与金鲳鱼皮ASC有相似的电泳性质和红外峰型,初步鉴定为I型胶原蛋白。该实验为金鲳鱼鱼皮酶溶性胶原的提取提供了理论参考。To improve the utilization rate of fish skin in processing by-product of Golden pompano, this paper used the response surface Box- Behnken method to optimize the extraction process of pepsin- soluble collagen （ PSC ） from the skin of Golden pompano.Based on the previous single factor experiment, the model was established by three factors （enzyme concentration, liquid-to-solid ratio and extraction time）.In addition, the basic properties of PSC were characterized and compared with acid- soluble collagen（ASC） by Sodium Dodecyl Sulfate Poly Aclylamide Gel Electrophoresis （SDS-PAGE）, Attenuated Total Reflectance Fourier Transform Infrared spectroscopy （ ATR- FTIR ） and Differential Scanning Calorimeter （ DSC ）. The results showed that the optimum extraction conditions were 1.5% enzyme concentration,23：1 mL/g liquid-to-solid ratio, and 27.5 h extraction time.Under this condition, the actual yield of collagen of Golden pompano skin was 64.68 %, which was close to the predicted value（65.17% ）.Moreover, the PSC obtained in this experiment had higher denaturation temperature （30.44 ℃ ） and preliminarily identified as type I collagen, which was similar to the electrophoretic and infrared peaks of ASC.The technical reference for the extraction of PSC from Golden pompano skin was given in this research.

关 键 词：金鲳鱼鱼皮 胶原蛋白 响应面法 提取工艺 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]