红发夫酵母有机硒最佳转化条件的研究  

Optimum Transformation Conditions of Organic Selenium by Phaffia rhodozyma

作　　者：徐洲 朱文优 魏琴 黎维 张超 

机构地区：[1]宜宾学院生命科学与食品工程学院固态发酵资源利用四川省重点实验室,四川宜宾644000

出　　处：《安徽农业科学》2017年第27期17-19,22,共4页Journal of Anhui Agricultural Sciences

基　　金：四川省重点实验室项目(NJ2014-10;2015GTY009;04JY029-102);四川省教育厅/实验室项目(09ZC051;2009KFJ001);四川省高校科研创新团队建设资助项目(14TD0031)

摘　　要：[目的]优化红发夫酵母(Phaffia rhodozyma)有机硒转化条件。[方法]通过正交试验研究硒添加量、培养时间和装液量对红发夫酵母富硒的影响。[结果]红发夫酵母富硒的最适培养基为PDA培养基,亚硒酸钠的添加方式为分2次添加,在硒添加量20 mg/L、培养时间30 h、装液量80 m L/500 m L的条件下,红发夫酵母的生物量达10.62 g/L,有机硒转化率达63.2%。[结论]研究结果为红发夫酵母富硒的进一步研究提供了参考。[Objective] The aim was to optimize the transformation conditions of organic selenium by Phaffia rhodozyma.[Method]The effect of selenium addition,culture time and liquid loading on selenium accumulation in Phaffia rhodozyma was studied by orthogonal experiment. [Result] The optimum medium for selenium enrichment was PDA medium,and the adding method was added in two times. Under the condition of selenium content at 20 mg/L and the liquid loading at 80 m L/500 m L incubating for 30 h,the biomass and transformation rate of organic selenium could reach 10. 62 g/L and 63. 2%,respectively.[Conclusion]The results can be regarded as the reference for further research on selenium enrichment in Phaffia rhodozyma.

关 键 词：红发夫酵母 有机硒 转化添加 生物量 

分 类 号：S-03[农业科学]