一株蝉拟青霉产N^6-(2-羟乙基)腺苷静置发酵条件的研究  

Static liquid fermentative conditions for producing N^6-(2-hydroxyethyl) adenosine of Paecilomyces cicadae

作　　者：雷帮星 康冀川 何劲[2] 文庭池 钱一鑫 何珺 

机构地区：[1]贵州大学西南药用生物资源教育部工程研究中心,贵州贵阳550025 [2]贵阳学院,贵州贵阳550005

出　　处：《菌物学报》2017年第10期1415-1426,共12页Mycosystema

基　　金：贵州省社发攻关项目[黔科合SY字(2015)3004];贵州省科学技术基金[黔科合J字(2012)2102号]~~

摘　　要：为了提高蝉拟青霉122菌株中N^6‐(2‐羟乙基)腺苷(HEA)的产量。采用静置液体培养方法,以HEA产量为指标,筛选得到122菌株在查氏培养基上的HEA产量优于沙氏和PD培养基;以查氏培养基为基础培养基,在26℃培养40d时,HEA产量达到最大;在黑暗条件培养优于光照条件培养;最优碳源是蔗糖和葡萄糖;最优氮源是磷酸氢二铵;最优无机盐是KH_2PO_4;最优前体物为次黄嘌呤;最适氨基酸为L‐谷氨酸。正交实验结果表明蔗糖是影响122菌株产HEA的最主要的因子,在筛选出的优化培养基上得到122菌株HEA的最大产量为(130.22±0.60)mg/L,较优化前HEA的产量提高9.73倍。The static liquid fermentation conditions of Paecilomyces cicadae strain 122 producing N^6-（2-hydroxyethyl） adenosine （HEA） were optimized. The detection of HEA was conducted by HPLC method. The results displayed that Czapek medium was the best one for producing HEA, and the yield was higher than that of Sabouraud and PD medium. The yield of HEA in Czapek medium at 26℃ for 40 days reached the maximum. Dark conditions were better than light conditions for cultivation of P. cicadae The optimal carbon sources were sucrose and glucose. The optimal nitrogen sources and inorganic salts were respectively diammonium phosphate and KH2PO4. The optimal additive and amino acid were respectively hypoxanthine and L-glutamic acid The results of orthogonal test showed that the most important factor for producing HEA of strain 122 was sucrose. Under the optimal condition, the highest yield of HEA of the strain was （130.22±0.60）mg/L, increased by 9.73 times over the condition prior to optimization.

关 键 词：蝉拟青霉 N^6-(2-羟乙基)腺苷 静置发酵 前体物 氨基酸 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]