玉米生物活性肽膜提纯工艺技术研究  

Study on membrane purification technology of corn bioactive peptide

作　　者：陆启明 陈志成[1] 

机构地区：[1]河南工业大学,郑州450001

出　　处：《粮食与食品工业》2017年第5期41-45,共5页Cereal & Food Industry

基　　金：国家自然科学基金项目(项目编号:31171789)

摘　　要：玉米蛋白粉在一定的温度、pH、底物浓度的条件下,经碱性蛋白酶水解后得到含有玉米低聚肽的混合液体,其中含有分子量大于1 280 Da的肽、蛋白及分子量小于260 Da的肽、氨基酸、各种离子,影响了低聚肽的纯度。采用透过分子量10 000 Da的超滤膜除去大分子物质,采用透过分子量1 000 Da的钠滤膜去除小分子物质及各种离子,得到分子量在260~1 280 Da的低聚肽,其液体含量在80%以上;该液体经浓缩至30%~35%,进行离心喷雾干燥,得到水分小于6%且低聚肽含量在80%以上的低聚肽粉。The corn protein powder,under the condition of certain temperature,Ph,substrate concentration,can get mixed liquid of corn oligopeptide by alkaline protease hydrolysis,which contains the peptide and protein,with molecular weight1 280 Da,and contains the peptide,amino acid,various ion with molecular weight 260 Da,which affects the purity of oligopeptide.The ultrafiltration membrane through10 000 Da molecular weight was used to remove macromolecules material and the ultrafiltration membrane through1 000 Da molecular weight was used to remove micromolecule material and various ion,then the oligopeptide is get with molecular weight 260-1 280 Da,and its liquid content 80%;the liquid is concentrated to 30% -35%,dried by centrifugal spray,the oligopeptide powder is obtained with liquid content 6%and oligopeptide content 80%.

关 键 词：玉米生物活性肽 超滤膜 钠滤膜 提纯 

分 类 号：TS201[轻工技术与工程—食品科学]