凝结芽孢杆菌13002高密度培养  

High cell density culture of Bacillus 13002

作　　者：孙丽娜 金迅 周全兴 周劲松[1] 刘冬梅[1] SUN Li- na JIN Xun ZHOU Quan- xing ZHOU Jin- song LILT Dong- mei(Institute of Food Science and Engineering,South China University of Technology,Guangzhou 510640, China College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China)

机构地区：[1]华南理工大学食品科学与工程学院,广东广州510640 [2]浙江大学生物系统工程与食品科学学院,浙江杭州310058

出　　处：《食品工业科技》2017年第21期114-120,共7页Science and Technology of Food Industry

基　　金：国家自然科学基金资助项目(31101254);广东省科技计划项目(2013B020312002;2014A020208019)

摘　　要：本实验旨在通过优化培养条件提高凝结芽孢杆菌13002发酵培养液中的菌体浓度,为生产高光学纯度L-乳酸奠定基础。通过单因素实验,最陡爬坡实验,Box-Behnken响应面实验确定凝结芽孢杆菌13002的最优培养基配方和最佳培养条件,并于自动发酵罐中进行高密度分批补料培养。结果表明,最优培养基配方为:葡萄糖20 g/L、细菌学蛋白胨10 g/L、酵母提取粉25 g/L、Na Cl 10 g/L,初始p H6.5。在此优化条件下,以6%的接种量,培养温度45℃,培养至16 h,以0.5 g/(L·h)速率添加补料至结束,最大菌体浓度达5.399 g/L,活菌总数为3.095×10~9CFU/m L,最终冻干后达0.730×10^(11)CFU/g。This experiment aimed to improve the concentration of bacteria in Bacillus 13002 fermentation medium by improving the culture conditions, and to lay the foundation for the production of high optical purity L-lactic acid.The single factor test, steep climbing test and Box-Behnken response surface test were used to confirm the optimum culture medium and optimum culture conditions of Bacillus 13002 and high density batch feeding was carried out in automatic fermentation tank. Results showed that the optimal medium was glucose 20 g/L, bacterial peptone 10 g/L, yeast extract powder 25 g/L, NaC1 I0 g/L, initial pH6.5.Under the optimum condition,when 6% Bacillus 13002 and nutrients were added at the rate of 0.5 g/（ L· h）from the 16th hour to the terminal at 45 ℃, the maximal cell concentration was 5.399 g/L.The total number of bacteria was 3.095 × 10^9 CFU/mL, and reached the level of 0.730× 10^11 CFU/g after lyophilization.

关 键 词：凝结芽孢杆菌13002 高密度培养 响应面 分批补料培养 

分 类 号：TS201.3[轻工技术与工程—食品科学]