凋亡蛋白酶活化因子1及半胱氨酸天冬氨酸蛋白酶9在卵巢浆液性癌中的表达及临床意义  

Expressions of Apaf-1 and Caspase-9 in ovarian serous carcinoma and their clinical significance

作　　者：武鑫磊 李金梅[1] 张丙信[1] 张金库[1] Wu Xinlei Li Jinmei Zhang Bingxin Zhang Jinku(Pathology Department, the First Central Hospital of Baoding, Baoding 0710001 China)

机构地区：[1]河北省保定市第一中心医院病理科,071000

出　　处：《中国综合临床》2017年第8期673-677,共5页Clinical Medicine of China

基　　金：河北省医学科学研究重点课题计划（20150404）

摘　　要：目的 探讨凋亡蛋白酶活化因子1（Apaf-1）和半胱氨酸天冬氨酸蛋白酶9（Caspase-9）在卵巢浆液性癌（OSC）中的表达及临床意义.方法 采用免疫组化SP法检测45例OSC,60例卵巢浆液性囊腺瘤和32例卵巢交界性浆液性囊腺瘤中Apaf-1和Caspase-9蛋白的表达,分析两者的表达与OSC临床病理特征的关系以及Apaf-1和Caspase-9在OSC中表达的相关性.结果 Apaf-1在OSC、囊腺瘤和交界性囊腺瘤表达的阳性率分别为24.4%（11/45）、75.0%（45/60）和46.9%（15/32）,差异有统计学意义（χ2=26.734,P〈0.01）.Apaf-1与OSC的病理分级、临床分期及淋巴结转移有关（χ2值分别为6.318、7.565、5.554,P均〈0.05）;Caspase-9在OSC中、囊腺瘤和交界性囊腺瘤的表达率分别为28.9%（13/45）、83.3%（50/60）和56.3%（18/32）,差异有统计学意义（χ2=31.682,P〈0.01）.Caspase-9与OSC的病理分级、临床分期及淋巴结转移有关（χ2值分别为5.750、4.391、5.466,P均〈0.05）.在OSC中Apaf-1和Capase-9的表达呈正相关（k=0.433,P=0.003）.结论 Apaf-1和Caspase-9在OSC中低表达,可能与OSC的发生、发展有关.Objective To investigate the expressions of Apaf-1 and Caspase-9 protein in ovarian serous carcinoma（OSC） and their clinicopathological significance.Methods The expressions of Apaf-1 and Caspase-9 protein in 45 cases of OSC,60 cases of ovarian serous cystadenomas and 32 cases of ovarian borderline serous cystadenomas were detected by immunohistochemical SP method.The relationship between the expressions of these two proteins and the clinicopathological features of OSC and the correlation between Apaf-1 and Caspase-9 expressions in OSC were analyzed.Results The positive rates of Apaf-1 in OSC,ovarian serous cystadenoma and ovarian borderline serous cystadenoma were 24.4%（11/45）,75.0%（45/60） and 46.9%（15/32）,the difference was statistically significant （χ2=26.734,P〈0.01）.Apaf-1 expression was correlated with pathological grade,clinical stage and lymph nodes metastasis （χ2=6.318,7.565,5.554,P〈0.05）.The expression rates of Caspase-9 in OSC,ovarian serous cystadenoma and ovarian borderline serous cystadenoma were 28.9%（13/45）,83.3%（50/60） and 56.3%（18/32）,the difference was statistically significant （χ2=31.682,P〈0.01）.The expression of Caspase-9 was correlated with pathological grade,clinical stage and lymph nodes metastasis （χ2=5.750,4.391,5.466,P〈0.05）.In OSC,the expressions of Apaf-1 and Capase-9 were positively correlated （k=0.433,P=0.003）.Conclusion The low expressions of Apaf-1 and Caspase-9 in OSC may be related to the occurrence and development of OSC.

关 键 词：凋亡蛋白酶活化因子1 半胱氨酸天冬氨酸蛋白酶9 卵巢浆液性癌 免疫组化 APAF-1 CASPASE-9 

分 类 号：R737.31[医药卫生—肿瘤]