UPLC-MS/MS法测定凉茶饮料中的有机酸和黄酮类化合物  

Quantitative Analysis of Organic Acid and Flavonoid in Herbal Tea by UPLC-MS/MS

作　　者：韩婉清 王斌 王莉 冼燕萍 

机构地区：[1]广州质量监督检测研究院,国家加工食品质量监督检验中心(广州),广州市食品安全检测技术重点实验室,广州市食品安全风险动态监测与预警研究中心,广东广州510000

出　　处：《食品研究与开发》2017年第21期130-134,共5页Food Research and Development

基　　金：广东省质监局科技计划项目(2015CZ13);广州市质监局科技计划项目(2015kj01)

摘　　要：建立一种超高效液相色谱-三重四级杆串联质谱法同时测定凉茶饮料中5种有机酸和黄酮类化合物的方法。样品经高速离心,甲醇稀释后,经BEH C18(100 mm×2.1 mm,1.7μm)色谱柱分离,乙腈-0.1%甲酸水(体积分数)梯度洗脱,串联质谱电喷雾负离子扫描,多反应监测(MRM)模式检测。5种目标物的方法检出限在0.2 mg/L^0.5 mg/L,在0.02 mg/L^0.5 mg/L浓度范围内,线性相关系数均大于0.999 0。添加水平为1.0 mg/L^40 mg/L时,平均回收率为86.3%~102%;相对标准偏差(RSD,n=6)为2.4%~8.7%。A method for quantitative analysis of 5 kinds of organic acid and flavonoid compounds in herbal tea by ultra high performance liquid chromatography tandem mass spectrometry （ UPLC-MS/MS） was developed. Samples were centrifuged, diluted with methanol, and separated on BEH C18 （100 mm×2.1 mm, 1.7 μm）col- umn with acetonitrile-0.1 % formic acid solution as mobile phase by gradient elution. Quantitative analysis was performed by a triple quadrupole MS system with negative electrospray ionization （ESI-） operated under multi-ple reaction monitoring （MRM） mode. The method showed a good linearity with correlation coefficients （r） larg-er than 0.999 0 at the concentration of 0.02 mg/L-0.5 mg/L. The limits of detection （LOD） of the method ranged 0.2 mg/L-0.5 mg/L. At the spiked level of 1.0 mg/L-40 mg/L,the average recoveries presented 86.3 %-102 % , with the relative standard deviation （RSD, n=6） values varied between 2.4 %-8.7 %.

关 键 词：凉茶 有机酸 黄酮类化合物 超高效液相色谱-串联质谱(UPLC-MS/MS) 

分 类 号：O657.63[理学—分析化学] TS275.2[理学—化学]