乳液颗粒度对中性蛋白酶酶解反应影响的研究  

Effect of particle size of emulsion on enzymatic hydrolysis reaction of 1398 neutral protease

作　　者：俞明[1] 钱俊青[1] 龚峰[1] 郭辉[1] 

机构地区：[1]浙江工业大学药学院,杭州310014

出　　处：《中国油脂》2017年第10期43-46,共4页China Oils and Fats

摘　　要：探索了1398中性蛋白酶酶解大豆乳液的反应速率受乳液颗粒度的影响。以1398中性蛋白酶在温度45℃、pH 7.0、加酶量120 U/m L(以乳液计)条件下,发现随着筛网目数(80~160目)增大,即颗粒度减小,其反应速率逐渐增大,200目与160目的乳液参与酶促反应的速率相近。根据反应起始至5 h内反应速率随乳液颗粒度变化的实验数据,拟合了不同酶解时间段乳液颗粒度与反应速率的关系式,由关系式曲线与实验值符合情况可知,在反应1 h之内及4~5 h之间,乳液颗粒度对反应速率影响的规律基本一致,而在反应1~4 h间为另一种规律。根据拟合关系式,可指导和优化大豆水相酶法提取大豆油的工艺。The effect of particle size of emulsion on the reaction velocity of 1398 neutral protease hydrolyzing soybean emulsion was studied. The enzymatic hydrolysis reaction of 1398 neutral protease was carried out at 45 ℃,pH 7. 0 and enzyme dosage 120 U/m L（ based on emulsion）. It was found that the reaction velocity increased with particle size increasing from 80 to 160 meshes（ particle size decreasing）,and the reaction velocity of 200 meshes emulsion was approximate with 160 meshes emulsion. According to experimental data of velocity change with particle size from reaction beginning to 5 h,the equation of particle size of emulsion with reaction velocity in different reaction time was fitted. Comparing the equation curve with experimental data,the law of particle size of emulsion influencing on reaction velocity in 1 h was consistent with that in 4-5 h,while it was different from that in 1-4 h. Based on the fitted formulas,it could direct and optimize the process of extraction of oil from soybean by aqueous enzymatic method.

关 键 词：蛋白酶 反应速率 颗粒度 大豆乳液 水相酶法 

分 类 号：TS221[轻工技术与工程—粮食、油脂及植物蛋白工程]