Differential expression of a cDNA clone in human liverversus hepatic cancer-highly homologous to aryl-dialkyl-phosphatase  

作　　者：WANG KAN KAN DA FANG WAN XIAO KUN QIU PEI XIN LU JIAN REN GU(National La6oratory for Oncogenes and Related Genes Shanghai Cancer Institute, Shanghai 200032)(Qidong Cancer Institute, Jiangsu 226200) 

出　　处：《Cell Research》1997年第1期79-90,共12页细胞研究（英文版）

摘　　要：We applied the technique of mRNA differential display to normal liver tissue and hepatoma cell line Hep3B. One of the isolated cDNA clones was expressed in human normal liver tissue but not in the human hepatocarcinoma cell line. Northern Blot analysis confirmed that high level of mRNA was expressed in human normal liver tissue but the level was decreased in non-cancerous liver tissue from hepatoma patients. Low level or no expression was observed in human hepatoma tissue. One of these transcripts was about 1.8 kb in length. Southern Blot analysis showed that it was a single copy gene. We obtained a full length cDNA clone of 2,395 bp by screening human liver 5’-stretch plus cDNA library. Nucleotide sequence indicated that this clone was highly homologous to aryldialkyl-phosphatase and possessed two polymorphic sites. Aryl-dialkyl--phosphatase which has a prominent role in the metabolism of several toxic, synthetic compounds, may be potentially related to human hepatocarcinoma susceptibility. The biological significance of its differential expression in normal versus malignant tissue is discussed.

关 键 词：HEPATOCARCINOMA mRNA DD aryl-dialkyl-phosphatase 

分 类 号：R735.7[医药卫生—肿瘤]