The apoptosis of HEL cells induced by hydroxyurea  

作　　者：GUI CHANG YUN CHU JIANG HENG YUE XIE RUO LAN QIAN(Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031) 

出　　处：《Cell Research》1997年第1期91-97,共7页细胞研究（英文版）

摘　　要：Hydroxyurea has been used to synchronize cultured cells to S-phase and used to treat patients with sicklecell anemia. Recently, we found that hydroxyurea can induce the apoptosis of HEL (human erythroleukemia) cells.The induced HEL cells showed ultrastructurally chromatin condensation with regular crescents at the nuclear edges and apoptotic bodies. However, the cells of K562, another human erythroleukemia cell line, did not show such morphological changes. Under fluoroscope, the HEL cells after induction often displayed a clear reduction in nuclear diameter and nuclear chromatin cleavage and condensation and the presence of nuclear ring and apoptotic bodies. Analysis with flow cytometry showed that the percentage of apoptotic cells is about 30-40% after HEL cells were induced by hydroxyurea for 3 days. DNA ladder can be observed by electrophoretic analysis.

关 键 词：HEL cells HYDROXYUREA APOPTOSIS 

分 类 号：R73－36[医药卫生—肿瘤]