Phosphorylation and biosynthesis of high molecular weight proteins of tumor nuclear matrix  

作　　者：BAZARNOVA TM TV BULDYAEVA LS FILATOVA SB AKOPOV IB ZBARSKY(NK Koltzov Institute of Developmental Biology, Russian Academy of Sciences, 26 Vavilov Street, 117344 Moscow,Russia) 

出　　处：《Cell Research》1998年第3期195-207,共13页细胞研究（英文版）

摘　　要：Our previous studies showed a predominance of high molecular weight protein group in tumor nuclear matrices. Contrary to normal cells, proteins of this group are preferentially phosphorylated. Phosphoproteins of hepatoma nuclear matrix are selectively subjected to rapid proteolysis. By alkali treatment and a monoclonal antibody against phosphotyrosyl residue the presence of two high molecular weight bands of phosphotyrosyl-containing proteins was detected in nuclear matrices of tumor but not of normal liver cells. High molecular weight protein group of tumor nuclear matrices revealed also a rapid turnover and preferential incorporation of labeled amino acids selectively inhibited by chloramphenicol.

关 键 词：Animals Antigens  Nuclear Hela Cells Humans Male MICE Mice  Inbred C57BL Mice  Inbred CBA Molecular Weight Neoplasms Nuclear Matrix Nuclear Proteins PHOSPHOPROTEINS PHOSPHORYLATION Phosphotyrosine RATS Rats  Wistar Research Support  Non-U.S. Gov't 

分 类 号：R730.2[医药卫生—肿瘤] Q279[医药卫生—临床医学]