青海茶卡盐湖嗜盐碱芽胞杆菌资源分析  

Analysis of halophilic and alkaliphilic Bacillus resources from Qinghai Chaka Salt Lake

作　　者：王小英[1] 刘国红[2] 刘波[2] 陈峥[2] 潘志针[2] 朱育菁[2] 王阶平[2] 唐建阳[2] WANG Xiaoying LIU Guohongz LIU Bo CHEN Zheng PAN Zhizhen ZHU Yujingz WANG Jieping TANG Jianyang(College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian 350116, China Agrieuhural Bio-resouree Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China)

机构地区：[1]福州大学生物科学与工程学院,福建福州350116 [2]福建省农业科学院农业生物资源研究所,福建福州350003

出　　处：《福州大学学报（自然科学版）》2017年第5期761-769,共9页Journal of Fuzhou University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(31370059);福建省农业科技重大专项基金资助项目(2015NZ0003);福建省种业创新与产业化工程资助项目(FJZZZY-1544);福建省种业创新资助项目"福建芽胞杆菌资源保藏中心";福建省自然科学基金资助项目(2016J01129);福建省公益资助项目(2015R1018-7);青年英才计划资助项目(YJRC2015-17)

摘　　要：采用3种不同的培养基,通过可培养法,从青海茶卡盐湖样品中共分离获得110株嗜盐碱芽胞杆菌.基于16S rRNA基因序列相似性鉴定及系统发育分析,得出这些菌株为芽胞杆菌10个属的41个种,它们与最近匹配模式菌株的16S rRNA基因序列相似性在96%~100%之间,以芽胞杆菌属为优势菌,共计25种83株;存在6个潜在新种.3种酶筛实验结果表明,87%的嗜盐碱芽胞杆菌菌株具有产酶能力,产蛋白酶70株,产纤维素酶81株,产木聚糖酶50株.青海茶卡盐湖中的芽胞杆菌种类丰富多样且能产多种酶,并且潜藏着新的微生物物种,研究结果可为嗜盐碱芽胞杆菌资源开发提供理论基础.110 halophilic and alkaliphilic Bacillus strains were isolated and cultured using three medium.Based on 16S rRNA gene similarities and phylogeny analysis,it was indicated that these halophilic and alkaliphilic Bacillus strains belonged to 10 genera including 41 species with 16S rRNA gene96%~100% similarity to the their closed type strains.The genus Bacillus was the dominant resource in Chaka Salt Lake,which include 25 Bacillus species including 83 strains.6 strains may represent the potential new species.From the three enzymes screening,it was showed that approximately 87%strains could produce enzymes,among which 70 strains produced protease,81 strains produced cellulose and 50 strains produced xylanase.The research indicated that there was not only an abundant diversity of Bacillus,which could produce many kinds of enzymes,but also some potential new microbial species in Qinghai Chaka Salt Lake,which provided basis for reasonable development of halophilic and alkaliphilic Bacillus resources.

关 键 词：嗜盐碱芽胞杆菌 蛋白酶 纤维素酶 木聚糖酶 青海省 茶卡盐湖 

分 类 号：Q939[生物学—微生物学]