高效液相色谱法测定果酒中的糖、甘油和乙醇  

Determination of sugar,glycerol and ethanol in ratafee with high performance liquid chromatography

作　　者：黄海涛[1] 杨力佳[1] 丁中涛[2] 李忠[1] 

机构地区：[1]云南烟草科学研究院烟草化学室,云南昆明650106 [2]云南大学化学系,云南昆明650091

出　　处：《云南大学学报（自然科学版）》2002年第5期375-377,共3页Journal of Yunnan University(Natural Sciences Edition)

摘　　要：研究了高效液相色谱法测定果酒中的糖、甘油和乙醇的方法 .果酒样品用Sep -Pak -C18固相微萃取小柱预分离 ,以WatersSugar-PakⅠ钙型阳离子交换柱为固定相 ,0 .0 5 g/LEDTA钙钠水溶液为流动相 ,示差折光仪为检测器 ,一次进样测定果酒样品中的蔗糖、葡萄糖、果糖、甘油和乙醇 .最低检测限在微克级 ,相对标准偏差在 0 .92 %～ 1.5 2 %之间 ,标准回收率在 97%～ 10 5 %之间 .方法用于果酒样品的测定 。A high performance liquid chromatography(HPLC) method for the determination of sugar,glycerol and ethanol in ratafee was studied.Ratafee was preseparated by solid phase extraction with Sep Pak C 18 column.Sugars,glycerol and ethanol were separated on a Waters Sugar Pak Ⅰ(6.5?mm×300?mm) cations ion exchange column,with a refractive index detector system and 0.05?g/L EDTA calcium disodium salt solution as mobile phase.Sucrose,glucose,fructose,glycerol and ethanol could be simultaneously determined in a single injection.The limits of detection were:sucrose 1.5?μg/mL,glucose 2.0?μg/mL,fructose 2.0?μg/mL,glycerol 2.5?μg/mL and ethanol 2.5?μg/mL.The mean recoveries of sugar,glycerol and ethanol were between 97% and 105% and the relative standard deviations were between 0.92%and 1.52%.This method can be applied to the determination of sugars,glycerol and ethanol in ratafee with satisfactory result.

关 键 词：高效液相色谱法 果酒 糖 甘油 乙醇 含量测定 Sugar-PakⅠ阳离子交换柱 固定相 

分 类 号：TS262.7[轻工技术与工程—发酵工程] TS261.7[轻工技术与工程—食品科学与工程]