长白山茗葱挥发油成分分析  

Components of Volatile Oil From Allium victorialis L.

作　　者：才燕[1] 王克凤 董然[1] 刘晓嘉[1] 安佰义[1] 

机构地区：[1]吉林农业大学园艺学院,吉林长春130118 [2]长春科技学院长白山生态资源开发工程研究中心,吉林长春130600

出　　处：《北方园艺》2017年第21期140-145,共6页Northern Horticulture

基　　金：吉林省发改委产业技术研究与开发资助项目(2013-779);国家科技部星火计划资助项目(2012GA660002);2013年长春市农业先进实用技术的示范推广计划资助项目(14NK009)

摘　　要：以长白山茗葱为试材,采用顶空固相微萃取法对其不同部位的挥发油进行提取,通过GC-MS联用技术进行分析鉴定,利用峰面积归一化法计算各化合物的相对质量分数。结果表明:从茗葱根挥发油中得到37个色谱峰,共鉴定出33种化合物,其中2,3-去氢-1,8-桉叶素(50.616%)、甲基-2-烯丙基二硫醚(19.036%)质量分数相对较高;鳞茎挥发油中得到21个色谱峰,共鉴定出19种化合物,其中硫化丙烯(28.459%)、二烯丙基二硫(27.765%)质量分数较高;叶片挥发油中得到24个色谱峰,共鉴定出22种化合物,其中(Z)乙酸叶醇酯(55.910%)、乙酸反-2-己烯酯(9.183%)2种化合物质量分数较高。HSSPME GC-MS是一种可用于分析不同部位茗葱挥发油成分变化的简单可行的分析方法。Taking Allium victorialis L.as test material,the volatile oils extracted from the Allium victorialis L.by HS-solid-phase microextraction（ SPME） were studied. Chemical constituents were identified by GC-MS. The compound quantification was calculated using the area normalization method. The results showed that 37 chromatographic peaks separated well were found in the roots of the volatile oils; 33 compounds were identified in the oils of roots of Allium victorialis L.,including 2,3-Dehydro-1,8-cineole（ 50. 616%）,Disulfide,methyl2-propenyl（ 19. 036%）.From the volatile oils of bulbs,21 chromatographic peaks separated well were found in the respective GC total ion current chromatograms of oils. 19 compounds were identified in the oils of bulbs in cluding Thiirane,methyl（ 28. 459%）,Diallyl disulphide（ 27. 765%）; 24 chromatographic peaks separated well were found in the respective GC total ion current chromatograms of oils; 22 compounds were identified in the oils of lesves of Allium victorialis L. 3-Hexen-1-ol,acetate,（ Z）-（ 55. 910%） 2-Hexen-1-ol,acetate,（ E）-（ 9. 183%） were the main components.Headspace SPME-GC-MS is a simple sampling method for measuring the variations of main constituents in volatile fragrance from fresh Allium victorialis L.

关 键 词：长白山茗葱 挥发油 固相微萃取 气相色谱-质谱连用 

分 类 号：S633.901[农业科学—蔬菜学]