效应面法与酶法联用提取黄果槲寄生果实多糖的优化工艺研究  

Optimization of polysaccharides extraction process from Viscum coloratum (Kom.) Nakai f. lutescens kitagfruits by enzymolysis combined response surface method

作　　者：叶文斌[1] 樊亮[2] 王昱[1] 黄兆辉[1] 杨小录[1] 王让军 

机构地区：[1]陇南师范高等专科学校农林技术学院,甘肃成县742500 [2]陇南师范高等专科学校数信学院,甘肃成县742500

出　　处：《甘肃农业大学学报》2017年第5期135-141,共7页Journal of Gansu Agricultural University

基　　金：甘肃省自然科学基金项目(1107RJZK243);甘肃省教育厅项目(2015B-148);陇南师范高等专科学校重点项目(2014LSZK01001);全国第四次中药资源普查甘肃试点两当普查点子项目(2015GSZYZYPC-10);2016年陇南市科技计划项目(2016-09)

摘　　要：【目的】通过效应面法与酶法联用工艺提取黄果槲寄生果实中的活性物质多糖.【方法】采用效应面法和酶法联用对提取工艺条件进行优化,以含量为评价指标,用单因素方差分析法研究酶解温度、酶添加量和pH对提取黄果槲寄生果实多糖含量的影响.【结果】提取最佳优化条件为酶解温度50℃,酶添加量0.06g和pH值4.80,提取3次.【结论】获得了黄果槲寄生果实多糖提取的最佳优化工艺参数,该条件下黄果槲寄生果实多糖提取预测值为10.01%,验证值为9.85%,该工艺条件易于控制、工艺简单、成本低,黄果槲寄生果实酶解后多糖的含量最高为9.85%.【Objective】To extract active material polysaccharides using enzymolysis and response surface method fromViscum coloratum（Kom.）Nakai f.lutescens kitagfruits.【Method】The extraction conditions were optimized by enzymolysis and response surface method and the effect of enzymolysis temperature,enzyme added and pH on polysaccharides content extracted fromViscum coloratum（Kom.）Nakai f.lutescens kitag fruits was analyzed using one-way variance analysis.【Result】The optimal extraction conditions were as follows：extraction temperature 50℃,enzyme added 0.06 g,pH 4.80 and extraction for three times.【Conclusion】The optimal process parameters were obtained for extracting polysaccharides fromViscum coloratum（Kom.）Nakai f.lutescens kitagfruits,under the best conditions,the predictive values of polysaccharides content was 10.01%and compliance value was 9.85%.The extraction conditions were easily controlled,simple and low cost,and the polysaccharides was up to 9.85% after enzymolysis of Viscum coloratum（Kom.）Nakai f.lutescens kitagfruits.

关 键 词：效应面法 酶法 黄果槲寄生果实多糖 优化工艺 

分 类 号：TS201.2[轻工技术与工程—食品科学] R284.2[轻工技术与工程—食品科学与工程]