胃癌患者外周血CD8^+CD28^+与CD8^+CD57^+T细胞亚群的变化及临床意义  

The Changes and Clinical Significance of Peripheral Blood CD8^+CD28^+ and CD8^+CD57^+T Cell Subgroup in Gastric Cancer Patients

作　　者：陈耀平[1] 屈蕾 陈静[1] 王宁菊[1] 

机构地区：[1]宁夏医科大学总医院,银川750004 [2]宁夏医科大学临床医学院医学检验系,银川750004

出　　处：《宁夏医科大学学报》2017年第9期1003-1005,1011,共4页Journal of Ningxia Medical University

基　　金：宁夏回族自治区科技攻关项目

摘　　要：目的探讨胃癌患者外周血CD8^+T淋巴细胞表面CD28、CD57的表达变化及其临床意义。方法用流式细胞仪检测66例胃癌患者和23例健康外周血CD8^+T淋巴细胞表面CD28和CD57的表达情况。结果术前胃癌组外周血中CD8^+CD28^+T细胞百分率低于对照组(P=0.035)。胃癌患者手术前后CD8^+CD28^+和CD8^+CD57^+T细胞的百分率差异均无统计学意义(P>0.05)。不同临床分期胃癌患者的外周血CD8^+CD57^+T细胞百分率差异有统计学意义(P=0.017);淋巴结转移胃癌患者的CD8^+CD57^+T细胞百分率与未转移者差异无统计学意义(P=0.051);侵及胃壁全层胃癌患者的CD8^+CD28^+、CD8^+CD57^+T细胞百分率均高于未侵及胃壁全层者(P≤0.05)。结论外周血中CD8^+CD28^+T细胞百分率的减少可能与促进肿瘤的发生有关;外周血CD8^+CD57^+T细胞百分率增加可能促进肿瘤的局部浸润。Objective To investigate the different expression and clinical significance of peripheral blood CD8^＋T cells CD28 and CD57 in gastric cancer patients. Methods Peripheral blood T lymphocyte surface CD8^＋CD28^＋and CD8^＋CD57^＋expression were detected in 66 cases of patients with gastric cancer and 23 healthy controls using flow cytometry. Results The level of peripheral blood CD8^＋CD28^＋T cell percentage in preoperative gastric cancer patients was significantly lower than those in healthy controls（P=0.035）. There was no statistically significant difference for the peripheral blood T lymphocyte CD8^＋CD28^＋and CD8^＋CD57^＋percentages in pre and post operation patients with gastric cancer（P〈0.05）. The percentages of CD8^＋CD57^＋T cells in patients with different clinical stages showed a statistical difference（P=0.017）. The percentage of CD8^＋CD57^＋T cells is lover in gastric cancer patients with lymph node metastasis group than that of withant metastasis group（P=0.051）. The percentages of CD8^＋CD28^＋T cells and CD8^＋CD57^＋T cells was significantly higher in gastric cancer patients with invasion full-thickness stomach lining than that of no contamination full thickness stomach group（P≤0.05）.Conclusion The loss of CD8^＋CD28^＋T cells in peripheral blood may be associated with the occurrence of tumor.The increase of peripheral blood CD8^＋CD57^＋T cells may promote tumor local invasion.

关 键 词：胃癌 CD8 CD28 CD57 T淋巴细胞 

分 类 号：R735.2[医药卫生—肿瘤]