双歧杆菌的分离鉴定及保藏性能研究  

Isolation,Identification of Bifidobacterium and Preservation Ability Research

作　　者：乌云[1] 刘红霞[1] 李洪亮[1] 母智深[1] 

机构地区：[1]内蒙古蒙牛乳业(集团)股份有限公司,内蒙古呼和浩特011500

出　　处：《食品研究与开发》2017年第23期171-176,共6页Food Research and Development

基　　金：内蒙古科技项目(201502056)

摘　　要：为丰富发酵剂及益生菌菌种资源,从内蒙古阿拉善盟采集7份新生婴儿粪便样品,在传统细菌分离方法的基础上,通过添加莫匹罗星锂盐和X-Gal(5-溴-4-氯-3-吲哚-β-D-半乳糖苷)改良基础培养基,快速有效地分离获得双歧杆菌。结合全自动微生物分析系统和API20A试剂条鉴定菌种。结果共分离获得6株双歧杆菌,其中3株动物双歧杆菌,2株青春双歧杆菌,1株短双歧杆菌。试验验证甘油浓度为70%时冷冻保藏效果最佳。In order to enrich more types of leavening agents and probiotics resources,faeces collected from seven infants(Alashan,Inner Mongolia)were investigated. On the basis of traditional bacterial separation method,Mupirocin lithium salt and X-Gal were added to the basic medium,to separate the bifidobacterium effectively.Automatic microorganism analytical system and API20 A reagent strip were applied to identify the bifidobacterium. In total,six strains of bifidobacterium were obtained from infacts' faeces,among which three strains were Bifidobacterium lactis,two strains were Bifidobacterium adolescentis,and one strain was Bifidobacterium breve,and the optimum storage condition for the bifidobacterium is 70 % glycerol.

关 键 词：双歧杆菌 全自动微生物分析系统 API20A试剂条 冷冻保藏 

分 类 号：TS201.3[轻工技术与工程—食品科学]