不同基因型核桃雄花营养品质及抗氧化活性分析  

Analysis of nutritional quality and antioxidant activity of male flowers in walnut

作　　者：李雪[1] 王长雷 史斌斌[1] 潘学军[1] 张文娥[1] 

机构地区：[1]贵州大学.贵州省果树工程技术研究中心,贵阳550025

出　　处：《果树学报》2017年第11期1394-1400,共7页Journal of Fruit Science

基　　金：贵州省高层次创新型人才培养项目[黔科合人才(2016)4038]

摘　　要：【目的】分析5个不同基因型核桃雄花营养品质及抗氧化活性的差异,为高效、合理利用丰富的核桃雄花资源提供参考。【方法】以贵州省赫章县核桃产区5个不同基因型盛花期的核桃雄花为试材,测定9种常规营养成分含量、9种矿质元素含量、17种氨基酸含量以及3种抗氧化活性物质和其抗氧化能力。【结果】(1)‘黔核7号’在常规营养成分和矿质营养方面优于其他基因型核桃雄花,‘黔核5号’和‘香玲’在矿质营养方面表现较差,而不同基因型核桃雄花在必需氨基酸和总氨基酸含量上并未达到差异显著水平,但在EAA/TAA的比例上‘黔核7号’(42.01%)和‘黔核8号’(41.98%)最高,‘香玲’(39.40%)最低。(2)不同基因型核桃雄花中‘黔核8号’生物活性成分含量最高,且抗氧化能力最强;‘黔核5号’生物活性成分含量最低,抗氧化能力最差。【结论】不同基因型核桃雄花在营养成分的组成上并无显著差异,而在含量上原产于贵州的核桃品种具有明显优势,尤以‘黔核7号’品质突出,‘香玲’品质相对较差。[Objective] In order to utilize the abundant resources of male flowers of walnut （Juglans regia）, nutritional quality and antioxidant activity of the male flowers of five different genotypes were evaluated. [ Methods ] The male flowers of ＇Qianhe-5＇ ＇Qianhe-7＇ ＇Qianhe-8＇ ＇Caishen- 10＇ and ＇Xiangling＇ were collected from Hezhang county of Bijie city, Guizhou province. The content of crude protein was determined by semi micro Kjeldahl method, the content of fat by Soxhlet extraction, the conent of soluble sugar by anthrone colorimetric method, titratable acid content by acid hydrolysis method, the content of starch, crude fibre and ash by acid hydrolysis, weighing and furnace method, separately. The content of mineral elements was measured by atomic absorption spectrophotometer. Determination of amino acids by automatic amino acid analyzer, the aseorbic acid by high performance liquid chromatography （HPLC）, to- tal phenol content by Folin Shaw colorimetry, total flavonoids content by aluminum nitrate colorimetric method, DPPH free radical scavenging test was carried out with reference to the method described by Mo- tamed, FRAP iron ion reduction test with reference to the method described by Hatamnia. The variance analysis and correlation analysis were carried out using DPS v7.05 software. The multiple comparisons were performed using the Duncan＇s new bipolar difference method. [ Results ]There were significant differ- ences in the contents of the normal nutritional components of male flowers among the different genotypes of walnut. All conventional nutrients except for dry matter had some differences, the content of total solu- ble sugar and starch had the highest difference. The order of the total soluble sugar content was ＇ Qianhe5＇ 〉 ＇ Qianhe-7＇ 〉 ＇ Qianhe-8＇ 〉 ＇Xiangling＇ 〉＇ Caishen- 10＇ and the order of the starch was ＇ Qianhe- 8＇ 〉 ＇ Qianhe-7＇ 〉 ＇Xiangling＇ 〉 ＇ Qianhe-5＇ 〉 ＇ Caishen- 10＇. The conventional nutrient content o

关 键 词：核桃 雄花 基因型 营养品质 抗氧化活性 

分 类 号：S664.1[农业科学—果树学]