大孔树脂纯化红腰豆总黄酮的工艺优化及其体外抗氧化活性比较  

Optimization of Purification Process of Total Flavonoids From Red Kidney Beans with Macroporous Absorbent Resin and Comparision of in vitro Antioxidant Activity

作　　者：黎英[1] 曾珍清 张薇[1] 郑兰香[1] 李子月[1] 陈雪梅[1] 

机构地区：[1]龙岩学院福建省家畜传染病防治与生物技术重点实验室,龙岩364012

出　　处：《中国粮油学报》2017年第11期128-136,143,共10页Journal of the Chinese Cereals and Oils Association

基　　金：龙岩学院产学研项目(LC2015011)

摘　　要：以红腰豆总黄酮粗提液为原料,研究大孔树脂对红腰豆黄酮的纯化工艺和效果,比较了8种树脂对红腰豆总黄酮的静态吸附和解吸性能,对AB-8型大孔树脂分离纯化红腰豆总黄酮进行了单因素、BoxBenhnken中心组合设计和响应面法优化试验,并考察了红腰豆总黄酮纯化前后体外抗氧效果。结果表明:AB-8树脂为纯化红腰豆总黄酮的最佳树脂,其最佳的吸附工艺条件为:上样质量浓度4.0 mg/m L,上样液pH 6.3,上样流速2.0 m L/min,上样体积5.0 BV,在此条件下吸附率可达(98.03±0.30)%;最佳的解吸工艺条件为乙醇体积分数75%,洗脱流速3.0 m L/min,洗脱体积2.0 BV,在此条件下解吸率可达(94.52±0.24)%。纯化后红腰豆总黄酮纯度提高了约2.85倍,纯化前DPPH·、·OH和O-2·的清除率IC50值分别为1.18、1.40、6.51 mg/m L,纯化后分别为0.37、0.82、1.77 mg/m L,纯化后红腰豆总黄酮提取物的体外抗氧化活性明显增强。The process and results of macroporous resins for the purification of flavonoids from red kidney beans were studied, and the performances of static adsorption - desorption of the eight kinds of resin were compared by using adsorption rate and desorption rate as indexes. Single factor test and Box - Behnken central composite design were conducted with response surface methodology to analyze and optimize the conditions for AB -8 macroporous resin separation and purification of total flavonoids from red kidney beans, and in vitro antioxidant effects before and after the purification of red kidney beans total flavonoids were studied. The results showed that the optimum purification resin was AB - 8. The best adsorption conditions were determined as 4. 0 mg/mL sample concentration, pH 6. 3, loading velocity 2. 0 mL/min, sample volume 5.0 BV, under which the adsorption rate could reach （98.03 ± 0. 30） %. Besides, the best desorption conditions were determined as 75% ethanol concentration, desorption velocity 3.0 mL/min and eluant volume 2. 0 BV, under which the desorption rate could get to （94. 52 ± 0. 24） %. The total flavonoids purity of red kidney beans increased about 2. 85 times after purification. Before purification, clearance IC50 values of 1,1 - di- phenyl - 2 - trinitrobenzene hydrazine （DPPH） radical scavenging, hydroxyl radical（ · OH） and superoxide anion radi- cal（ O2- · ） were 1.18,1.40,6. 51 mg/mL, respectively, which increased to 0. 37,0. 82,1.77 mg/mL after purification. After purification,in vitro antioxidant activity of red kidney bean total flavonoids extract significantly enhanced.

关 键 词：红腰豆 总黄酮 大孔树脂 响应面 抗氧化活性 

分 类 号：TS202.1[轻工技术与工程—食品科学]