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作 者:雷昕 李楠洋 汤雨凌 刘龙洲[1] 田光明[1] 杨烨[1]
机构地区:[1]长江大学动物科学学院,湖北荆州434025 [2]湖北省荆州中学,湖北荆州434020
出 处:《畜牧与兽医》2017年第11期24-29,共6页Animal Husbandry & Veterinary Medicine
基 金:湖北省自然科学基金重点项目(2013CFA100);国家自然科学基金(31472117)
摘 要:为了研究超微茶粉对生长猪生长性能、胴体品质及脂肪代谢的影响,将64头50 kg左右的杜长大阉公猪随机分为4组,每组4栏(重复),每栏4头,对照组采食基础日粮,试验组分别添加0.5%、1.0%和1.5%的超微茶粉,试验期70 d,试验结束时计算日增重、料重比,然后屠宰测定分析肌肉胴体品质及脂肪沉积性能。结果表明,超微茶粉对生长猪生长性能没显著影响,并且能显著降低肌内脂肪(IMF)含量,可以显著提高生长猪的瘦肉率和眼肌面积、猪肉肉色以及降低滴水损失和剪切力(P<0.05)。同时,利用前体脂肪细胞研究表没食子儿茶素没食子酸酯(EGCG)对脂肪代谢关键基因表达的影响,结果表明,前体脂肪细胞在EGCG作用下,p-AMPKα蛋白和LPL基因表达显著增加,同时C/EBPβ、SREBP-1、PPARγ基因表达显著降低(P<0.05)。我们的结果提示,超微茶粉对生长猪生长性能没有影响,但可以显著改善肌肉品质,其作用机理可能与对脂肪代谢的调控有关。The experiment was conducted to explore the effects of superfine grinding tea(SGT) on growth performance,carcass quality and lipid metabolism of growing pigs.Sixty-four DLY pigs with initial body weight of(46.9±2.6) kg were randomly allocated into 4 groups of 4 replicates with 4 pigs in each replicates.Group 1 was control with basal diet.The experimental groups were fed by the basal diet adding0.5%,1.0%,and 1.5% superfine grinding tea.The experiment was lasted for 70 day.The daily weight gain and feed to gain ratio were calculated and carcass quality and lipid metabolism were analyzed.The results showed that the superfine grinding tea had no significantly effects on growth performance,but significantly decreased IMF contents and increased muscular color,loin eye area,and lean percentage(P〈0.05).Meanwhile,the superfine grinding tea significantly decreased the dropping loss and shear force of meat(P〈0.05).On the other hand,the isolated subcutaneous preadipocytes were conducted to study the effects of EGCG on key gene related to lipid metabolism.The results showed that the EGCG inhibited the expression of C/EBPβ,SREBP-1 and PPARγ and promoted the expression of LPL genes and pAMPKα proteins(P〈0.05).The results suggested that superfine grinding tea supplement had not influence on the growth of growing pigs,while improved the carcass quality.The action mechanism of superfine grinding tea might be related with its regulating effects on lipid metabolism.
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