基于铁蛋白-壳聚糖制备芦丁纳米复合物及芦丁稳定性研究  

Research on Preparation of Rutin Nanocomposite based on Ferritin-Chitosan and Stability Analysis of Rutin

作　　者：杨瑞[1,2] 田婧[2] 刘玉茜 周中凯[1,2] 

机构地区：[1]天津科技大学新农村发展研究院,天津300457 [2]教育部食品营养与安全重点实验室天津科技大学食品工程与生物技术学院,天津300457

出　　处：《中国食品学报》2017年第9期58-65,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31501489);天津市自然科学基金项目(16JCQNJC14500);天津市十三五综投项目(2017KDYB02)

摘　　要：芦丁是一种常见的具有重要生理作用的黄酮类活性物质,其水溶性差且不稳定,限制了在食品领域的应用。本文以分离纯化的重组H-2铁蛋白(r H-2)和壳聚糖为原料,利用尿素诱导的铁蛋白可逆组装性质和铁蛋白-壳聚糖的相互作用,制备均匀分散的水溶性r H-2-壳聚糖-芦丁包埋物。采用HPLC法测定1分子的r H-2铁蛋白可包埋约23.8个芦丁分子,其包埋率和装载率分别为15.8%和2.59%。相比游离的芦丁分子,r H-2-壳聚糖-芦丁包埋物中芦丁分子的紫外辐射和加热处理的稳定性分别提高了44.7%和28.9%。本研究成果对提高芦丁在食品加工过程中的稳定性具有重要意义。Rutin is a common dietary flavonoid with important pharmacological activities. However, its application in the food industry and medical field is limited mainly because of its poor water-solubility and instability towards light and thermal treatment. In this paper, by using purified recombinant H-2 ferritin （rH-2） as material, rH-2-rutin complex was prepared, followed by ferritin-chitosan interaction, resulting in water soluble rH-2-chitosan-rutin composite. It was cal- culated that about 23.8 of rutin molecules were entrapped in the ferritin cage, and the embedding rate and loading rate were 15.8% and 2.59%, respectively. Furthermore, compared with free rutin, the light stability and thermal stability of rutin in rH-2-chitosan-rutin was significantly improved by 44.7%- 28.9%, respectively. Results obtained from this paper will benefit the further application of rutin in food industry.

关 键 词：铁蛋白 芦丁 壳聚糖 包埋 稳定性 

分 类 号：TQ28[化学工程—有机化工]