预防性给予生物活性肽对巨噬细胞抵御LPS刺激的调节作用  

Regulatory Effect of Pre-Feeding Bioactive Peptide to RAW264.7 under LPS Stimulation

作　　者：胡亚菁 高扬[1] 李锡安 梁世排 陈静[1] 李婉如[1] 张少辉[1,3] 吴金鸿[1] 

机构地区：[1]上海交通大学农业与生物学院,上海200240 [2]浙江熊猫乳业集团股份有限公司,浙江温州325800 [3]上海交通大学陆伯勋食品安全研究中心,上海200240

出　　处：《上海交通大学学报（农业科学版）》2016年第6期14-20,共7页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：国家自然科学基金项目(31491623)

摘　　要：预防性给予巨噬细胞(RAW264.7细胞)不同浓度的生物活性肽Gln-Glu-Pro-Val(QEPV)后,用脂多糖(LPS)刺激细胞,通过检测细胞因子表达和炎性蛋白基因转录,观察生物活性肽QEPV对细胞抵御LPS刺激的调节作用。结果表明,0.1g/L的QEPV有显著的促进细胞增殖作用,0.5g/L浓度以下的QEPV对RAW264.7细胞无增殖抑制作用。0.5g/L QEPV预处理RAW264.7细胞后,用LPS刺激细胞,其IL-6、IL-12等促炎细胞因子的分泌降低,抗炎细胞因子IL-10分泌提前,分泌量增加,COX-2、iNOS基因的转录水平降低,说明乳源性生物活性肽QEPV对RAW264.7细胞抵御LPS刺激起正向调节作用。Pre-feeding different concentrations of bioactive peptide Gln-Glu-Pro-Val(QEPV)was used to evaluate its regulatory effects on the responds of macrophage(RAW264.7)under lipopolysaccharide(LPS)stimulation.The results show that 0.1g/L QEPV has positive effect on cells reproduction and QEPV below 0.5g/L has no reproduction suppression effect on RAW264.7cells.Pre-feeding 0.5g/L QEPV can suppress RAW264.7cells to produce IL-6/IL-12 and promote to produce IL-10.QEPV can suppress the expression of COX-2/iNOS gene in transcription level.The research shows that the bioactive peptide QEPV has positive regulatory effect on protecting RAW264.7cells from LPS stimulation.

关 键 词：生物活性肽 巨噬细胞 脂多糖 炎症 

分 类 号：Q51[生物学—生物化学]