脉冲场凝胶电泳(PFGE)技术在一起肠炎沙门氏菌食源性疾病暴发中的溯源分析  

PFGE together Salmonella enteritidis traceability analysis of foodborne illness outbreaks

作　　者：缪国忠 章剑[1] 何政[1] 徐超[1] 张宏宾[1] 

机构地区：[1]江阴市疾病预防控制中心,江苏214400

出　　处：《医学动物防制》2017年第12期1288-1289,1292,共3页Journal of Medical Pest Control

基　　金：江苏省卫生行业研究专项(201302005)

摘　　要：目的对一起食源性疾病暴发事件调查中采集的样本进行致病菌检验,使用脉冲场凝胶电泳(Pulsed-field gel electrophoresis,PFGE)技术对菌株分子流行病学检测以确定引起暴发事件病原菌间的关联性。方法依据GB 4789方法对采集的样品进行病原菌分离、鉴定及药敏试验(K-B法),应用PFGE检测技术对检出的肠炎沙门氏菌进行分子分型,通过BioNumerics软件进行聚类分析。结果从4份病人肛拭子标本、1份剩余三明治和1份操作台擦拭物中均分离出肠炎沙门氏菌,经酶切后PFGE电泳聚类图谱相似性100%。结论经PFGE分子分型分析,确认这是一起由食用了肠炎沙门氏菌污染的食物后引起的食源性疾病。PFGE可有效应用于食物中毒溯源分析及分子流行病学研究。Objective To conduct a microbiological test on samples collected from a food poisoning event, in which PFGE was applied for the detection of strains to identify the pathogens which caused food poisoning. Methods According to GB 4789, the isolation, identification and K - B test were conducted on collected samples, and the isolated Salmonella enteritidis strains were detected by PFGE molecular typing（grouping） and were analyzed by Bio -numerics software to perform clustering. Results 4 patients from stool specimens and 2 Salmonella enteritidis was isolated in the environmental samples, after enzyme digestion, 6 strains of bacteria have identical PFGE electrophoresis, PFGE clustering atlas similarity analysis is 100%. Conclusion Confirmed by the PFGE molecular classification analysis, it is by eating together enteritis caused by Salmonella enteritidis contamination of food after foodborne illness outbreaks, PFGE can be used in the source tracing and molecular epidemiological study of food poisoning.

关 键 词：食源性疾病 肠炎沙门氏菌 脉冲场凝胶电泳 

分 类 号：R516.3[医药卫生—内科学]