辣椒素抑制Lewis肺癌小鼠血管生成的实验研究  

Inhibitory effect of capsaicin on angiogenesis in Lewis lung cancer of mice

作　　者：李华洋[1] 许婧[1] 任公平[1] 姜爱英[1] 

机构地区：[1]牡丹江医学院红旗医院,黑龙江牡丹江157011

出　　处：《中国现代医学杂志》2017年第29期6-10,共5页China Journal of Modern Medicine

摘　　要：目的观察辣椒素对Lewis肺癌小鼠血管生成的影响,并探讨其可能的机制。方法复制Lewis肺癌小鼠模型,随机分为模型组,辣椒素低、中、高剂量组,以及阳性对照组5组,每组9只。各组连续用药20 d,于末次给药后处死全部小鼠,比较各组小鼠瘤体积、瘤重、抑瘤率及微血管密度(MVD)。采用免疫组织化学法检测CD34阳性细胞率;采用Western blot和实时荧光定量聚合酶链反应检测缺氧诱导因子1α(HIF-1α)、血管内皮生长因子(VEGF)蛋白和m RNA表达的变化。结果与模型组比较,辣椒素抑制Lewis肺癌小鼠瘤体积、瘤重、MVD和CD34阳性细胞率(P<0.05);与模型组比较,辣椒素抑制Lewis肺癌小鼠HIF-1α、VEGF蛋白和m RNA的表达(P<0.05)。结论辣椒素抑制Lewis肺癌小鼠肿瘤生长和血管生成,其机制可能与抑制HIF-1α和VEGF的表达有关。Objective To investigate the effect of capsaicin on angiogenesis in Lewis lung cancer of mice, and to discuss its possible mechanism. Methods The mice with transplanted Lewis lung cancer were randomly divided into 5 groups （9 in each）： model group, low-dosage capsaicin group, medium-dosage capsaicin group, high-dosage capsaicin group, and positive control group. After continuous medication for 20 days, the mice were sacrifced. The tumor volume, tumor weight, tumor inhibitory rate and microvessel density （MVD） were compared among the 5 groups. The rate of CD34＋ cells was detected by immunohistochemical analysis. The mRNA and protein expressions of hypoxia-inducible factor-1α （HIF-1α） and vascular endothelial growth factor （VEGF） were detected by qRT-PCR and Western blot respectively. Results Capsaicin signifcantly inhibited the tumor volume and weight, MVD and the rate of CD34＋ cells compared with the model group （P 〈 0.05）. Capsaicin also signifcantly inhibited the mRNA and protein expressions of HIF-1α and VEGF in the Lewis lung cancer of mice compared with the model group （P 〈 0.05）. Conclusions Capsaicin can inhibit tumor growth and angiogenesis in Lewis lung cancer of mice which might be related to the down-regulation of HIF-1α and VEGF.

关 键 词：辣椒素 肺癌 血管生成 缺氧诱导因子1Α 血管内皮生长因子 

分 类 号：R734.2[医药卫生—肿瘤]