大孔吸附树脂法分离纯化红景天中洛塞维  

The isolation and purification of Rosavin by the macroporous adsorption resin

作　　者：杨清林[1] 葛静秋 

机构地区：[1]重庆科技学院化学化工学院,重庆401331

出　　处：《食品工业科技》2017年第23期79-84,91,共7页Science and Technology of Food Industry

摘　　要：本文采用大孔吸附树脂法对红景天中洛塞维(Rosavin)分离纯化进行了研究,建立了如下工艺条件:Rosavin检测的高效液相(High performance liquid chromatography,HPLC)最佳的流动相为乙腈-0.2%磷酸(65/43,v/v),最佳柱温为30℃;选用非极性树脂D312,最大吸附量上样浓度为0.24 mg/m L,装柱高度为30 cm,粗品最大上样量为4 BV,上样流速为1~2 BV/h,洗脱剂为50%乙醇溶液。大孔吸附树脂分离纯化目的物Rosavin的含量由3.00%提高到了68.76%。大孔吸附树脂法分离纯化洛塞维的工艺条件合理,节约成本、收率高,适合工业应用。To establish a method for the Rosavin of isolation and purification, the result of process conditions were as follows： the conditions of HPLC test is acetonitrile-0.2% phosphoric acid （65/43, v/v）of mobile phase, and column temperature condition 30℃ ;on the comparison of six resin＇ s adsorption and the establishment of the resin were preliminary isothermal static adsorption curve, we can determined the optimal resin was D312 resin, and the largest loading concentration was 0.24 mg/mL;column height 30 cm,sample size 4 BV,flow rate 1-2 BV/h.The results showed that the Rosavin content had increased from 3.00% to 68.76% , based on the consideration of four factors in column chromatography process. The macroporous adsorption resin of isolation and purification of Rosavin is reasonable, it is suitable for industrial applications.

关 键 词：红景天 洛塞维 高效液相 大孔吸附树脂 分离纯化 

分 类 号：TS201.1[轻工技术与工程—食品科学]