一种大鼠尺骨在体运动疲劳损伤模型的构建  

Construction of an in vivo athletic fatigue damage model of rat ulna

作　　者：李昊[1] 李瑞欣[1] 韩标[1] 韦淑萍 苏卫华[1] 秦晓丽[1] 安慰宁[1] 吴航 张西正[1] 

机构地区：[1]军事医学科学院卫生装备研究所,天津300161 [2]武警后勤学院解剖学教研室,天津300162

出　　处：《生物医学工程与临床》2017年第6期571-575,共5页Biomedical Engineering and Clinical Medicine

基　　金：国家自然科学基金重点项目(11432016);国家自然科学基金面上项目(31370942;31470935)

摘　　要：目的提出一种大鼠尺骨运动疲劳损伤模型的在体建立方法,为后续骨组织运动疲劳损伤特点及修复重建的实验研究提供平台和依据。方法选择3个月龄雌性Wistar大鼠36只,体质量约250 g。利用前期文献查阅、有限元计算分析和大鼠离体尺骨疲劳力学性能测定结果,对大鼠尺骨组织施加在体疲劳加载,建立在体运动疲劳损伤模型。从微观-宏观多个层面检测疲劳加载后骨组织细胞的响应、显微损伤情况及骨组织力学性能的变化,验证模型的有效性。结果按照确定的运动疲劳加载条件(1.5 Hz,3 000με,8 000次/2天),经过1周在体疲劳加载,大鼠尺骨割线模量和循环能量耗散值下降显著,尺骨中段截面制片中观察到显微裂纹,空白骨细胞陷窝的比例(40.96%±4.88%)显著升高,破骨细胞活性显著增强(约为对照组的1.45倍),成骨细胞活性相对减弱(约为对照组的87%)。从多个层面证明骨组织疲劳和显微损伤的出现,进而验证了大鼠尺骨在体运动疲劳损伤模型的成功建立。结论成功建立并验证了一种大鼠尺骨在体运动疲劳损伤模型,可以为军队士兵或中长跑运动员运动疲劳损伤预防与治疗的相关研究提供动物模型和理论支持。Objective To construct an in vivo athletic fatigue damage model of rat ulna, and provide the basis for subsequent researches on bone athletic fatigue damage and the associated bone targeted remodeling. Methods Thirty-six female Wistar rats aged 3-month were selected. Based on previous references, results of finite element analysis（FEA） and in vitro fatigue mechanical property test of rat ulna, the Wistar rats were fatigue loaded to establish in vivo athletic fatigue damage model. Multilevel detections of cellular response, micro-damage and mechanical properties were conducted for model verification. Results After 7-day in vivo fatigue loading determined as 1.5 Hz, 3 000 με, 8 000 cycles/2 days, the secant modulus of rat ulna and cyclic enery dissipation decreased significantly, microcracks were observed in ulna middle section, while the proportion of empty osteocyte lacunae（40.96 % ± 4.88 %） was significantly increased. Osteoclast activity was significantly enhanced（about1.45 times of control group） and osteoblast activity was relatively weakened（about 87 % of control group）. The bone tissue fa-tigue and emergence of micro-injury of rat ulna was confirmed by multi-level verification, and the successful establishment of rat ulnar body fatigue injury model was validated. Conclusion An in vivo athletic fatigue damage model of rat ulna is successfully established and verified, which could provide an animal model and theoretical reference for prevention and treatment of athletic fatigue damage in military recruits and middle-distance athletes.

关 键 词：高强度训练 运动疲劳损伤 在体加载 动物模型 

分 类 号：R-332[医药卫生] R68