酸角壳提取物对蔗糖负荷大鼠血糖及α-葡萄糖苷酶和α-淀粉酶的影响  

Effects of tamarind shell on sucrose tolerance and the activity of α-glucosidase and α-amylase in sucrose-loaded rats

作　　者：李维熙 刘冬丽[3] 苏薇薇[1] 高永坚[2] 

机构地区：[1]中山大学生命科学学院,广州510275 [2]国药集团广东环球制药有限公司 [3]云南中医学院中药学院

出　　处：《环球中医药》2017年第11期1218-1223,共6页Global Traditional Chinese Medicine

摘　　要：目的研究酸角壳提取物对蔗糖负荷大鼠糖吸收的抑制作用。方法采用蔗糖负荷大鼠模型观察酸角壳提取物对蔗糖负荷后不同时间点血糖的影响;采用酶标法测定酸角壳提取物对正常大鼠肠黏膜和内容物中α-葡萄糖苷酶和α-淀粉酶活性的影响,评价酸角壳提取物对肠道α-葡萄糖苷酶和α-淀粉酶活性的影响。结果各剂量酸角壳提取物能显著降低蔗糖负荷后15分钟大鼠的血糖水平(P<0.01)。酸角壳提取物能明显抑制小肠上段和中段α-葡萄糖苷酶以及小肠中段α-淀粉酶的活性,在给药30分钟到1小时的抑制作用最为显著。结论酸角壳提取物能够显著降低蔗糖负荷后大鼠的血糖水平,其作用可能与抑制肠道α-葡萄糖苷酶和α-淀粉酶活性有关。Objective To study the inhibitory effects of tamarind shell extract on intestinal absorption of sucrose. Methods The sucrose load model of rats were used and blood glucose levels were analyzed chronologically to evaluate the effect of tamarind extract. In vivo experiments were also performed to study the inhibitory effects of tamarind shell extract on the activities of α-glucosidase and α-amylase in the small intestinal mucosa and contents of rats. Results Tamarind shell extract of all doses had significant effects on the blood glucose levels after 15 min and 30 min of surcrose administration（ P〈 0. 01）. Tamarind shell extract also significantly inhibited the activities of α-glucosidase and α-amylase in the small intestinal mucosa and contents. Conculusion Tamarind shell extract could effectively reduce the blood glucose level in sucrose-loaded rats. The mechanism might be related to its inhibitory effects on the activities of α-glucosidase and α-amylase.

关 键 词：酸角壳提取物 糖负荷 Α-葡萄糖苷酶 Α-淀粉酶 

分 类 号：R285.5[医药卫生—中药学]