NF-κB信号通路蛋白在木脂素对胃癌SGC-7901细胞抗增殖作用中的机制研究  

Mechanism of NF-κB signaling pathway on the anti-proliferative effect of lignans on gastric cancer SGC-7901 cells

作　　者：焦璐[1] 王莹 吕游[1] 李柱虎[1] 

机构地区：[1]延边大学医学院,吉林延吉133002

出　　处：《时珍国医国药》2017年第10期2392-2395,共4页Lishizhen Medicine and Materia Medica Research

基　　金：吉林省科技厅科研项目(20140414050GH)

摘　　要：目的探讨NF-κB信号通路蛋白在木脂素对胃癌SGC-7901细胞抗增殖作用中的机制。方法利用MTT、流式细胞术和Hochest染色、western blot方法检测木脂素对胃癌SGC-7901细胞增殖、凋亡、细胞周期和NF-κB信号通路相关蛋白p50和p65蛋白及bax和bcl-2蛋白表达的影响。结果 MTT结果表明,木脂可抑制胃癌SGC-7901细胞的增殖,并具有浓度和时间依赖性(P<0.05或P<0.01);流式细胞结果表明,木脂素促进细胞凋亡,与对照组相比有显著性差异(P<0.05),具有浓度依赖性,并阻止胃癌SGC-7901细胞从G0/G1期进入S期;western blot结果,木脂素下调p65、p50和bcl-2蛋白的表达而上调bax蛋白的表达。结论木脂素抑制胃癌SGC-7901细胞的增殖和凋亡与NF-κB信号通路密切相关。Objective To investigate the mechanism of NF-κB signaling pathway in the antiproliferative effect of lignans on gastric cancer SGC-7901 cells. Methods The proliferation and apoptosis of gastric cancer SGC-7901 cells were detected by MTT,flow cytometry and Hochest staining. Western blot was used to detect the expression of p50 and p65 protein and bax and bcl-2 protein expression of the impact. Results MTT results showed that lignans could inhibit the proliferation of gastric cancer SGC-7901 cells and had a concentration and time-dependent（ P〈0. 05 or P〈0. 01）; The results of flow cytometry showed that lignan-induced apoptosis was significantly different from that of the control group（ P〈0. 05）,and the gastric cancer SGC-7901 cells were prevented from entering the S phase from G0/G1 phase. Western blot results showed that lavalin down-regulated the expression of p65,p50 and bcl-2 protein up-regulated bax protein. Conclusion Lignans inhibitied gastric cancer SGC-7901 cell proliferation and apoptosis may be closely related to NF-κB signaling pathway.

关 键 词：木脂素 胃癌细胞 NF-KB P65 P50 

分 类 号：R361.2[医药卫生—病理学]