牛磺酸对染锰大鼠神经氧化损伤的保护作用  

The protective effect of taurine on the nerve oxidative damage of rats exposed to manganese

作　　者：杨猛[1] 张霞霞 刘文瑜 刘舫[1] 

机构地区：[1]山东中医药大学附属医院,山东济南250000

出　　处：《工业卫生与职业病》2017年第6期410-413,共4页Industrial Health and Occupational Diseases

摘　　要：目的探讨牛磺酸对染锰大鼠神经氧化损伤的影响。方法选取Wistar大鼠45只,随机分为对照组、染锰组和牛磺酸组,每组各15只。对照组腹腔注射(ip)生理盐水30 mg/kg,每日1次,共20次;染锰组ip注射15 mg/kg MnCl_2·4H_2O溶液,1次/d,共20次;牛磺酸组在染锰组基础上,皮下注射牛磺酸125.15 mg/kg,隔日1次共注射10次。进行Morris水迷宫试验、跳台试验,分别检测大鼠丘脑中谷氨酰胺酶、谷氨酸脱羧酶、谷氨酰胺合成酶含量,超氧化物歧化酶(SOD)、丙二醛(MDA)含量。结果染锰组与对照组比较,染锰组SOD明显降低[(35.57±3.26)μg/L]、MDA含量明显增高[(230.32±35.28)nmol/g],Morris水迷宫的潜伏期[(41.2±3.1)s)明显延长、错误次数[(5.4±2.5)次]明显增加,跳台试验潜伏期第1天[(55.6±7.8)s]、第2天[(49.7±9.3)s]、第3天[(42.8±8.7)s]、第4天[(35.7±8.4)s]均明显延长,谷氨酰胺酶C(0.72±0.08)μg/L]、谷氨酸脱羧酶[(107.64±8.54)ng/L]、谷氨酰胺合成酶[(142.68±28.96)ng/L]含量均明显降低,差异有统计学意义(P<0.01)。牛磺酸组与染锰组比较,牛磺酸组SOD[(39.47±4.15)μg/L]含量明显增高,MDA含量[(203.05±32.53)nmol/g]明显降低,Morris水迷宫潜伏期[(30.5±2.9)s]明显缩短,错误次数[(3.9±1.4)次]明显减少,跳台试验潜伏期第1天[(46.7±7.4)s)、第2天[(38.6±8.9)s]、第3天[(33.7±7.8)s]、第4天[(27.6±6.9)s]均明显缩短,谷氨酰胺酶[(0.82±0.09)μg/L]、谷氨酸脱羧酶([(135.36±11.79)ng/L]、谷氨酰胺合成酶[(168.73±29.97)ng/L]含量均明显增高,差异均有统计学意义(P<0.01)。结论牛磺酸明显提高了染锰大鼠的学习记忆能力,改善了脑组织谷氨酸代谢,抑制了神经过氧化反应,对染锰大鼠神经氧化损伤具有保护作用。Objective To study effects of taurine on the nerve oxidation of rats with manganic exposure.Methods 45 Wistar rats were randomly divided into control group, manganese dye group and taurine group（15 rats each group）. In the control group,the rats were abdominally injected（IP） with 30 mg/kg dose of manganese, which was 20 times a day. In the manganese dye group,an IP injection of 15 mg/kg MnCl_2·4 H_2 O solution was administered at least 20 times a day.The taurine group,on the basis of dyeing manganese,was injected with taurine 125. 15 mg/kg subcutaneous,and injected 10 times in the next day. In Morris water maze experiment,diving platform experiment, rats was detected in the thalamus glutaminase,glutamic acid decarboxylase, glutamine synthetase content, superoxide dismutase SOD and malondialdehyde MDA content. Results Compared with the control group,the SOD in the dye manganese group was obviously lower[（35. 57 ±3. 26） mug/L]and the MDA significantly higher（230. 32 ± 35. 28）nmol/g. The Morris water maze of incubation period[（41. 2±3. 1）s],and the times the number of errors（5. 4±2. 5）in the dye manganese group was significantly prolonged. The platform incubation period in day 1[（55. 6 ± 7. 8） s],day 2[（49. 7±9. 3）s],day 3[（42. 8±8. 7）s],and the fourth day[（35. 7±8. 4）s]were significantly prolonged in the dye manganese group. The glutaminase[（0. 72±0. 08）mug/L）,glutamic acid decarboxylase[（107. 64±8. 54）ng/L],glutamine synthetase[（ 142. 68 ± 28. 96） ng/L]in the dye manganese group were significantly lower, and the difference was statistically significant（P 0. 01）. Compared with the dye manganese group,the SOD [ taurine group（ 39. 47 ± 4. 15） mug/L]in the taurine group significantly increased, the MDA[（203. 05±32. 53 nmol/g）] significantly lower. The Morris water maze incubation period[（30. 5 ± 2. 9） s],times the number of errors（3. 9 ± 1. 4） in the taurine group were significantly shortened. The platform incubation period day 1

关 键 词：牛磺酸 染锰大鼠 神经氧化损伤 保护 

分 类 号：R745.1[医药卫生—神经病学与精神病学]