高产γ-氨基丁酸菌株Lp-145筛选与鉴定  

Isolation and identification of Strain Lp-145 for high yield γ-aminobutyric acid

作　　者：褚洋洋[1] 隋春光[2] 

机构地区：[1]黑龙江林业职业技术学院,牡丹江157000 [2]黑龙江农业经济职业学院,牡丹江157000

出　　处：《中国食品添加剂》2017年第11期87-93,共7页China Food Additives

摘　　要：从泡菜汁中筛选出一株菌株L-145,该菌株γ-氨基丁酸产量4.752 g/L。菌株L-145经形态学鉴别、生理生化性质及分子生物学鉴定。通过提取菌株的16Sr DNA测序并Blast比对,通过系统发育树的构建,最终确定菌株L-145为植物乳杆菌并命名为Lp-145。菌株Lp-145具有较高的发酵底物产γ-氨基丁酸的能力,可继续优化发酵条件来提高γ-氨基丁酸产量。The strain L-145 was isolated from kimchi juice.The yield of γ-aminobutyric acid was 4.752 g/L.The strain L-145 classification was identified based on morphological characteristics,physiological and biochemical properties and molecular biology.By 16SrDNA sequencing and Blast alignment of the extracted strain,and phylogenetic tree construction,the strain L-145 was labelled as Lp-145 by Lactobacillus plantarum.The strain Lp-145 has a high fermentation substrate that can produce GABA,and the fermentation conditions can be continuously optimized to increase γ-aminobutyric acid production.

关 键 词：Γ-氨基丁酸 筛选 鉴定 

分 类 号：TQ922[轻工技术与工程—发酵工程] TS202.1