植物核苷二磷酸激酶研究进展  

Research Advances of NDPKs in Plants

作　　者：朱馨妮 汪珊珊[1] 周佳琴 朱世华[2] 

机构地区：[1]宁波大学海洋学院,宁波315211 [2]宁波大学科学技术学院,宁波315211

出　　处：《生物技术通报》2017年第11期19-28,共10页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31371595)

摘　　要：核苷二磷酸激酶(Nucleoside diphosphate kinases,NDPKs)是一类高度保守的蛋白,大小一般在70-100 k D,在生物体内大多数以六聚体形式存在,仅在少数原核生物中以四聚体形式存在。NDPK主要参与维持核苷二磷酸和核苷三磷酸的平衡。目前在植物中已发现4种NDPK:NDPKⅠ、NDPKⅡ、NDPKⅢ和NDPKⅣ,相关研究主要集中在前3种。NDPKⅠ与植物生长发育、非生物胁迫、感病应激和激素响应有关;NDPKⅡ参与光合作用和活性氧清除;NDPKⅢ参与能量代谢和细胞程序性死亡;NDPKⅣ仅在拟南芥和水稻基因组中发现,预测定位于内质网,功能未知。除了上述的主要作用外,NDPK在某些植物中还有特殊功能,如参与DNA复制、参与淀粉和纤维素的合成、参与生长素调节和发挥核酶活性等。这些作用机制是否存在物种特异性还有待进一步的研究。对NDPK的系统进化、生物功能的最新进展进行了综述。最后对NDPK的发展趋势进行了展望,有助于将来对NDPK进行更深入和全面的研究。Nucleoside diphosphate kinases（NDPKs）are a class of highly conserved proteins with a size between 70 to 100 k D. Theyexist as hexamers in most organisms while as tetramers only in few prokaryotes. NDPKs are mainly responsible for keeping the in vivo cellularbalance between nucleoside diphosphates and nucleoside triphosphates. Four types of NDPKs,i.e.,NDPK Ⅰ,NDPK Ⅱ,NDPK Ⅲ andNDPK Ⅳ,are found in plants,however,till now researches have been mainly focused on the first three. NDPKIs are found to be involved inplant growth and development,and response to abiotic stress,pathogens and hormones. NDPK Ⅱ s take part in photosynthesis and removalof reactive oxygen species. NDPKIIIs are involved in energy metabolism and programmed cell death. NDPKIVs are only found in Arabidopsisand rice genomes and predicted to be located in endoplasmic reticulum with unknown function. Besides,NDPKs also display special functionsin certain plants,such as DNA replication,starch and cellulose synthesis,auxin signaling and ribozyme activities;however,whether thesefunctions are species-specific needs further study. This article summarizes the phylogenetic analysis and the most recent research progresson biological functions of NDPKs. Finally,the article discusses the prospect of NDPKs research,thus it will facilitate the in-depth andcomprehensive study of their function in plants in the future.

关 键 词：核苷二磷酸激酶 NDPK 系统进化 功能 

分 类 号：Q946[生物学—植物学]