七叶一枝花种子萌发不同阶段贮藏物质及淀粉酶活性变化  

Carbohydrate Content and Amylase Activity in Germinating Seeds of Paris polyphylla Smith var. chinensis

作　　者：苏海兰[1,2] 周先治 李希[1] 陈敏健 卓景泰[1] 唐建阳[1] 肖凤友 齐诚龙[4] 

机构地区：[1]福建省农业科学院农业生物资源研究所,福建福州350003 [2]农业部福州热带作物科学观测实验站,福建福州350003 [3]福建省农业科学院农业生态研究所,福建福州350003 [4]福建承天农林科技发展有限公司,福建南平354100

出　　处：《福建农业学报》2017年第10期1145-1149,共5页Fujian Journal of Agricultural Sciences

基　　金：国家标准化项目(ZYBZH-Y-FJ-09);农业部公益性行业科研专项(201507002);国家工信部项目(2015年华重楼);福建省科技计划项目--省属公益类科研院所基本科研专项(2014R1018-11;2015R1018-13);福建省农业科学院创新团队建设项目(STIT2017-2-8)

摘　　要：探讨七叶一枝花种子萌发不同阶段内含物质及淀粉酶活性变化规律,为七叶一枝花种子休眠解除和提高成苗率提供科学依据。以不同萌发阶段七叶一枝花种子为研究材料,采用显微镜观察、生理生化和质谱测定方法,研究淀粉含量、可溶性糖含量、α-淀粉酶活性、β-淀粉酶活性、总淀粉酶活性差异。结果表明,种子生理休眠解除后,淀粉酶被激活、淀粉被水解,淀粉酶活性不断下降,种子内贮存的淀粉、可溶性糖含量也逐渐减少,α-淀粉酶活性对七叶一枝花种子休眠解除及随后的发芽中起着决定性的作用。The stored nutrients and amylase activity in the seeds of Paris polyphylla Smith var.chinensis at various stages of germination were determined in an attemptto improve seed germination and seedling emergence.The seeds were collected for morphological observations under a light microscope,as well as analyses of physiological,biochemical properties.Changes on the contents of starch and soluble sugars and the activities ofα-amylase,β-amylase and total amylase during the seed dormancy and germination were monitored.The results showed that,at cessation of dormancy,the amylases in the seeds were activated and started to degrade starches.As the stored carbohydrates decreased,the amylase activity also declined gradually.α-amylase activity played a pivotal role in the dormancy termination and subsequent germination ofthe seeds of P.polyphylla.

关 键 词：七叶一枝花 萌发阶段 淀粉 可溶性糖 淀粉酶活性 

分 类 号：R282[医药卫生—中药学]